Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204144_4n21 ATGGGGTCATACATTTCAGCTTCTTAGGAAGG(+CAGTGT)GTACCTACAAAGGTAGCAAAATATTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605204143_8ced GTACCCATGTACAATTATATTCACATTCTGAG(+CAGTGT)GTACCGGAGATTGGGGTCTCAGAGTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605204141_kw9i CCCAGATTTGTACTCTGAGACCCCAATCTCCG(+CAGTGT)GTACCTCAGAATGTGAATATAATTGTACATG NGG 10 8 17 8 24 0 100 5 10
    240605204140_g7zp TGCTCTGAGGACCCGTATGACGGGTACTCTGG(+CAGTGT)GTACCAGGCTACCAGGGACACACCAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605204138_lxcw AGAGCATCACTTGGTGTGTCCCTGGTAGCCTG(+CAGTGT)GTACCCAGAGTACCCGTCATACGGGTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605204137_pq6s GGTCACATCATCTTAACGTAAACATCAATAAG(+CAGTGT)GTACCAACATTGTTAATGGAGGAAAAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240605204135_n8md TTTGGGTTGCTTTTCCTCCATTAACAATGTTG(+CAGTGT)GTACCTTATTGATGTTTACGTTAAGATGATG NGG 10 8 17 8 24 0 100 5 10
    240605204134_0p6s TGTCACGCAGAATGTGGTGTCCTGACTCAGTG(+CAGTGT)GATCCCCGTGCCTGCCGGGATCTGTGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605204132_ii57 AAGTGGTGTTCACAGATCCCGGCAGGCACGGG(+CAGTGT)GATCCACTGAGTCAGGACACCACATTCTGCG NGG 10 8 17 8 24 0 100 5 10
    240605204131_38l3 TCTTTTCCTTAAGGTGACAGTACCATAGTAGG(+CAGTGT)GTACCCTTCTTTGCATGGTATGGAAGCATGA NGG 10 8 17 8 24 0 100 5 10