Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205859_i1g6 AGTGGCTGAGCTGCAGGGGCGGGGACAAGGTG(+CAGTGT)GATCCTTGGAGAGCATTCCTCCACTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605205856_mwd1 TTATAGCCTTTCGCCTCTCCTAGCTTCTCCCG(+CAGTGT)GATCCTCACCCCTACCCACACAACTTCATAT NGG 10 8 17 8 24 0 100 5 10
    240605205854_38d8 AAAGGATATGAAGTTGTGTGGGTAGGGGTGAG(+CAGTGT)GATCCGGGAGAAGCTAGGAGAGGCGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605205852_wr9y TTCCGTATGTTAGCTCAGTCTTGTATTCCTGG(+CAGTGT)GATCCATCCCATTTGGTCATGGTGTCTAATC NGG 10 8 17 8 24 0 100 5 10
    240605205850_dwhk AAAATGATTAGACACCATGACCAAATGGGATG(+CAGTGT)GATCCCAGGAATACAAGACTGAGCTAACATA NGG 10 8 17 8 24 0 100 5 10
    240605205849_4sg2 ACCCGGCCTGGCTCTTGTGTTTTGACTAATTG(+CAGTGT)GATCCCAGGTGCAAGCCTTGCTTGGTCACAA NGG 10 8 17 8 24 0 100 5 10
    240605205846_lkc8 AGGGGTTGTGACCAAGCAAGGCTTGCACCTGG(+CAGTGT)GATCCAATTAGTCAAAACACAAGAGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605205844_6pjj AACTACAGTTGTGGAATCAGACTGGCTGGCTG(+CAGTGT)GATCCCAGCTCTGCCCCTTTGGCATCATCTT NGG 10 8 17 8 24 0 100 5 10
    240605205843_nnp8 CACCTAAGATGATGCCAAAGGGGCAGAGCTGG(+CAGTGT)GATCCAGCCAGCCAGTCTGATTCCACAACTG NGG 10 8 17 8 24 0 100 5 10
    240605205841_0heg CTTTAACTGAGAAGCAGAGAGAGGGGCAAGTG(+CAGTGT)GATCCTTGGTTTACTCAGGGAATCCAGACCA NGG 10 8 17 8 24 0 100 5 10