Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204110_0zx4 TCAGAAGTCATGTTTCCAGCCTGATACATCCG(+CAGTGT)GATCCAGCGGCCAATTGACTTGGTGCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605204109_cimx ATTCTGTAGATCTGGCTAGCCTCAAACTCAGG(+CAGTGT)GATCCGCCCACCTCTGCCTCCCACGTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605204107_ng17 TAATTTCAGCACGTGGGAGGCAGAGGTGGGCG(+CAGTGT)GATCCCTGAGTTTGAGGCTAGCCAGATCTAC NGG 10 8 17 8 24 0 100 5 10
    240605204106_orx4 GCTATATTCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTAAGATGGCTGTGAGCCACCACG NGG 10 8 17 8 24 0 100 5 10
    240605204104_z2lz AACCACGTGGTGGCTCACAGCCATCTTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAT NGG 10 8 17 8 24 0 100 5 10
    240605204103_mq66 TGACCTAGTTAAATACAGGGTAGGAATGAGAG(+CAGTGT)GATCCCTCACCAGATGGAACAATCAGACTGA NGG 10 8 17 8 24 0 100 5 10
    240605204101_wz38 ACGTTTCAGTCTGATTGTTCCATCTGGTGAGG(+CAGTGT)GATCCTCTCATTCCTACCCTGTATTTAACTA NGG 10 8 17 8 24 0 100 5 10
    240605204100_asx6 CTGTTATTATTGAAGATCATTTCCACTCTACG(+CAGTGT)GTACCACAGGACACTTAAGACTCTTCACAAG NGG 10 8 17 8 24 0 100 5 10
    240605204058_f3a1 TGGCTCTTGTGAAGAGTCTTAAGTGTCCTGTG(+CAGTGT)GTACCGTAGAGTGGAAATGATCTTCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240605204056_fpid TGTTTGCCTTGTTCTCCAGGGGGGCTGCTGGG(+CAGTGT)GTACCCTGGCTTGGGGTGCCCTGTCTTGGTT NGG 10 8 17 8 24 0 100 5 10