Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205821_5cdm GGGAAATGGGCAGGGGGCATGAGTCTTCCATG(+CAGTGT)GTACCAGCGAGGCCGTGGACATAATTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605205820_i7ne GCACATGGATCTCACCCGTATTCTGTTGTATG(+CAGTGT)GTACCTCGGTACATCTTTGTGGTTGTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605205818_ry6v CAGTTTTAACACAACCACAAAGATGTACCGAG(+CAGTGT)GTACCATACAACAGAATACGGGTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240605205817_rce5 GAGTAAATAGGGGACATGGTGCATTTTTGCTG(+CAGTGT)GATCCTGTGAAAGGTTTATTTAGAGCCGATT NGG 10 8 17 8 24 0 100 5 10
    240605205815_tt2q TCTGGAATCGGCTCTAAATAAACCTTTCACAG(+CAGTGT)GATCCAGCAAAAATGCACCATGTCCCCTATT NGG 10 8 17 8 24 0 100 5 10
    240605205814_ppct TAGTTTTGAGGGCTTACACAAGCTTATTCTTG(+CAGTGT)GTACCACAGGAATTTGGTTAAATGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605205811_eujg CAAAGGAAATTCCATTTAACCAAATTCCTGTG(+CAGTGT)GTACCAAGAATAAGCTTGTGTAAGCCCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605205810_0n0j GCATTCCCCGCTGCAGTCACCTCATAGGCTTG(+CAGTGT)GATCCATCAAGACCTACTTCAGGCCCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240605205808_aoge TGGAGAGCCGGGGCCTGAAGTAGGTCTTGATG(+CAGTGT)GATCCAAGCCTATGAGGTGACTGCAGCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605205806_h7db AGCGCACAGGGAACAGAGGGAGGCCCACCAGG(+CAGTGT)GATCCTGGCTTGCCTACCGAAGGCTATTGCT NGG 10 8 17 8 24 0 100 5 10