Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204200_evpw CTGACATTCTGTGGGCTCATACCTATTTGGTG(+CAGTGT)GATCCTCAAGGTTTCTTCTGGATTGAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605204158_bjfu GGAGGCAGGAACGCTTTGAAAAAGGCAAACGG(+CAGTGT)GATCCACTGAACCTCATCTTTATCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605204157_sb6r CCTCCACCCAAGGATAAAGATGAGGTTCAGTG(+CAGTGT)GATCCCGTTTGCCTTTTTCAAAGCGTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605204156_8m6y CACGGGGTGGGGGTGAGGGAAGCAGGCAGGTG(+CAGTGT)GATCCCTGAGGCTCACTGGACAGCCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605204154_1zk0 TAGGTCAGGCTGGCTGTCCAGTGAGCCTCAGG(+CAGTGT)GATCCACCTGCCTGCTTCCCTCACCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605204153_unm0 CAGGAGGATCTCAAATTCAAGACCACTCTGGG(+CAGTGT)GTACCCAAGGAGACCCTGCCTCAAAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240605204150_7vgy ATATTTGTGCTTTTGAGGCAGGGTCTCCTTGG(+CAGTGT)GTACCCCAGAGTGGTCTTGAATTTGAGATCC NGG 10 8 17 8 24 0 100 5 10
    240605204149_deip TTGAATAAATAGTTCAGGCTTTTCTCATCTCG(+CAGTGT)GTACCCAGGCTCCTGGAGACTGAAGATCAAC NGG 10 8 17 8 24 0 100 5 10
    240605204147_dvqw GGGTAGTTGATCTTCAGTCTCCAGGAGCCTGG(+CAGTGT)GTACCGAGATGAGAAAAGCCTGAACTATTTA NGG 10 8 17 8 24 0 100 5 10
    240605204146_4gc9 CCCACACACACAATATTTTGCTACCTTTGTAG(+CAGTGT)GTACCCTTCCTAAGAAGCTGAAATGTATGAC NGG 10 8 17 8 24 0 100 5 10