Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204129_b3x6 AAGATTCATGCTTCCATACCATGCAAAGAAGG(+CAGTGT)GTACCCTACTATGGTACTGTCACCTTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605204127_gy86 CGCTTTGTCCTTATGTGAATCTGCTATTGCGG(+CAGTGT)GATCCAAGCATTTGAGGAATATGTATATAGA NGG 10 8 17 8 24 0 100 5 10
    240605204123_5c3a CATGTTCTATATACATATTCCTCAAATGCTTG(+CAGTGT)GATCCCGCAATAGCAGATTCACATAAGGACA NGG 10 8 17 8 24 0 100 5 10
    240605204121_m3p7 GTAGTGCCTGCAGATGCCAGAAGAGGGCGATG(+CAGTGT)GATCCTTTGGAAACAATGTTATTGGCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605204119_971d AACTCAAGCTGCCAATAACATTGTTTCCAAAG(+CAGTGT)GATCCATCGCCCTCTTCTGGCATCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605204118_dvp4 TGCTATGTAGTCCCAGCTGGCCTCAAACTCAG(+CAGTGT)GATCCTCCTGCCTCAGCCTAGAATGGAACTC NGG 10 8 17 8 24 0 100 5 10
    240605204116_c2ux CTTAAGAGTTCCATTCTAGGCTGAGGCAGGAG(+CAGTGT)GATCCTGAGTTTGAGGCCAGCTGGGACTACA NGG 10 8 17 8 24 0 100 5 10
    240605204115_irma ATTTCAGTTTAGGAAGTGTGCCGTTTCATTAG(+CAGTGT)GTACCAAAGCACTTGCACTTTTGGGAACATA NGG 10 8 17 8 24 0 100 5 10
    240605204114_8qwr ACACCTATGTTCCCAAAAGTGCAAGTGCTTTG(+CAGTGT)GTACCTAATGAAACGGCACACTTCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605204112_hoi6 AGAAATTGCTGCACCAAGTCAATTGGCCGCTG(+CAGTGT)GATCCGGATGTATCAGGCTGGAAACATGACT NGG 10 8 17 8 24 0 100 5 10