Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605205839_04an CTGGCTGGTCTGGATTCCCTGAGTAAACCAAG(+CAGTGT)GATCCACTTGCCCCTCTCTCTGCTTCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605205836_p0s8 ATAACCCCATGCTCTGGCCCTGGCATCCCAAG(+CAGTGT)GTACCACAAATGCCATCGGCCTTGCTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605205835_d028 CTTGCCACACAGCAAGGCCGATGGCATTTGTG(+CAGTGT)GTACCTTGGGATGCCAGGGCCAGAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605205833_mfkp CCTTTAAAGCTGTGTCCACCTGTCAGTACCAG(+CAGTGT)GTACCATGGCAACGGCGGCAGCCACTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605205831_e44m GGTAGGGGGAAGTGGCTGCCGCCGTTGCCATG(+CAGTGT)GTACCTGGTACTGACAGGTGGACACAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605205829_q1hq CGAGCTCCAGGGACTTGGTGTTGCTTGGGAGG(+CAGTGT)GATCCATACAGCATTTTGCGTTCTTCATGTG NGG 10 8 17 8 24 0 100 5 10
    240605205827_wzk2 GATTACACATGAAGAACGCAAAATGCTGTATG(+CAGTGT)GATCCCTCCCAAGCAACACCAAGTCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605205826_jj69 CTCCTACTTTGTTCCCAGTAGTCCCGCGTAGG(+CAGTGT)GATCCTGACCCGGCAGTGTGCTAAGCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605205824_a2n9 CTGGCCTCTTGCTTAGCACACTGCCGGGTCAG(+CAGTGT)GATCCCTACGCGGGACTACTGGGAACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605205823_p2vi CCGAGGGGGAAATTATGTCCACGGCCTCGCTG(+CAGTGT)GTACCATGGAAGACTCATGCCCCCTGCCCAT NGG 10 8 17 8 24 0 100 5 10