Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204229_5kuy ATTTACACAGCATCCCTGAAGCAGCCTGCCTG(+CAGTGT)GTACCTTCCAAGGTACATTGGCTATAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605204227_idj7 CCCGACCTATTTTGGTTTTGGGGGGCATACGG(+CAGTGT)GTACCTTTTGTGTTATTAGTTTATGTATATT NGG 10 8 17 8 24 0 100 5 10
    240605204226_g293 ATCTTAATATACATAAACTAATAACACAAAAG(+CAGTGT)GTACCCGTATGCCCCCCAAAACCAAAATAGG NGG 10 8 17 8 24 0 100 5 10
    240605204224_q7om TTTCAGTGGTATGTCAAGTACAAGGCCTCTGG(+CAGTGT)GATCCACACAAGCAGATGTTCTTGGAAATCA NGG 10 8 17 8 24 0 100 5 10
    240605204223_rjfo ATGGATGATTTCCAAGAACATCTGCTTGTGTG(+CAGTGT)GATCCCAGAGGCCTTGTACTTGACATACCAC NGG 10 8 17 8 24 0 100 5 10
    240605204221_x3dt AGGCCCAGGATCTAGATTGCAGAGAACTTGAG(+CAGTGT)GATCCATGACTTAGCCAGCAGGTGTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605204220_rat4 TGGAGCAAAACACACCTGCTGGCTAAGTCATG(+CAGTGT)GATCCTCAAGTTCTCTGCAATCTAGATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605204219_zb30 GGCCCTGCTCAGATGGATGCAGAGCATTGAGG(+CAGTGT)GATCCAGATGAGGTGTGGTCCAGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605204217_dqog GATGCCCTCTTCCCTGGACCACACCTCATCTG(+CAGTGT)GATCCCTCAATGCTCTGCATCCATCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605204215_z2t7 CCTAACCTTCCTTACCACACCAGCCTGGGACG(+CAGTGT)GATCCGAGCCCCAGCGGGAAGCCATTCTCTT NGG 10 8 17 8 24 0 100 5 10