Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204214_57qs CCTGGAAGAGAATGGCTTCCCGCTGGGGCTCG(+CAGTGT)GATCCGTCCCAGGCTGGTGTGGTAAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605204212_clbn CCAGCCCTTGACACAGTCCAGCTGTGCCACAG(+CAGTGT)GTACCACACTGGGAACTTGTCACCCTCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605204211_e7ng AAGTGTCTTGAGGGTGACAAGTTCCCAGTGTG(+CAGTGT)GTACCTGTGGCACAGCTGGACTGTGTCAAGG NGG 10 8 17 8 24 0 100 5 10
    240605204209_bk3p TTTAGCTCATAGACTAGCAGAGCCACCCTTAG(+CAGTGT)GATCCTTCCTCTCCCATGTTTACCCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605204208_2z6h CGGAGCATGCTGGGTAAACATGGGAGAGGAAG(+CAGTGT)GATCCTAAGGGTGGCTCTGCTAGTCTATGAG NGG 10 8 17 8 24 0 100 5 10
    240605204206_pk9e AGCTTGCCTCCTGATACCAAATTATAATTTTG(+CAGTGT)GATCCTTTAAGGATCTTACATTTAATTAATC NGG 10 8 17 8 24 0 100 5 10
    240605204205_nehf TAAATGATTAATTAAATGTAAGATCCTTAAAG(+CAGTGT)GATCCAAAATTATAATTTGGTATCAGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605204204_81xt AAGCTGAGCAAGGCAGGGGAAGCGAGCTTCTG(+CAGTGT)GATCCTGATGTTTTGTGCAGGAATAGAAATG NGG 10 8 17 8 24 0 100 5 10
    240605204203_csmd CTTAGCATTTCTATTCCTGCACAAAACATCAG(+CAGTGT)GATCCAGAAGCTCGCTTCCCCTGCCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605204202_ktsj AGCAGGAGGCTCAATCCAGAAGAAACCTTGAG(+CAGTGT)GATCCACCAAATAGGTATGAGCCCACAGAAT NGG 10 8 17 8 24 0 100 5 10