Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204040_75cw GGCCCAGTCTATGAGGAAGGAGTGGCCCCTGG(+CAGTGT)GTACCCAGGCCACTGGCTTCAGTGGCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605204039_bmj0 AAGGAACTTCTTAGATGGGCTTGTGATGTCAG(+CAGTGT)GTACCCCCCCACCCTTACAGAGCGCAGACAT NGG 10 8 17 8 24 0 100 5 10
    240605204036_eecv GGCTCATGTCTGCGCTCTGTAAGGGTGGGGGG(+CAGTGT)GTACCTGACATCACAAGCCCATCTAAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605204035_0b4i AGATCCTGCTTCTCATGGGACTTCGATGAATG(+CAGTGT)GATCCAGCCGCACAATCTACCCCTTCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605204033_v3c1 AGGGCACTGGGAAGGGGTAGATTGTGCGGCTG(+CAGTGT)GATCCATTCATCGAAGTCCCATGAGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605204031_erhq CATCAGGAGCGACACCAAACAGAGTGCGTGGG(+CAGTGT)GATCCCCAATTGGCCCAGTGTCCCCCGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605204030_g3hi AGGAAGGGCCGGGGGACACTGGGCCAATTGGG(+CAGTGT)GATCCCCACGCACTCTGTTTGGTGTCGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605204028_p4g6 AGGCCCCTCACAACCCTTCTGCTCCAAGAGGG(+CAGTGT)GATCCAGCAGGTGCCTCTGCCCAGGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605204026_6p5s GGAAGCCAGGCCCTGGGCAGAGGCACCTGCTG(+CAGTGT)GATCCCCTCTTGGAGCAGAAGGGTTGTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605204025_33k9 AACCTGATCAGAGCCTCCTTTCCTTGTTTTGG(+CAGTGT)GATCCTGAATGGGCAAGAGTCTGTCCCCTGT NGG 10 8 17 8 24 0 100 5 10