Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203930_pc04 CCCTGTGTACCCTCTGAAGTCACCCTTCCTTG(+CAGTGT)GTACCTATGTGGGGAGAGATTAGGCAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605203928_56sx CACACACCTGCTTCCTGGGTCCTATGGACAGG(+CAGTGT)GTACCCACCATACCCTTCCTATCCCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605203926_orh7 CCTGGGAGGGAGGGATAGGAAGGGTATGGTGG(+CAGTGT)GTACCCTGTCCATAGGACCCAGGAAGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605203925_4v75 GGTTGGCTTCGCTGTTTTCCGGTTCCGTTCCG(+CAGTGT)GATCCAGCCTAGGCCTCACACACACCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605203923_ovbg GCCCCTGCCCGGTGTGTGTGAGGCCTAGGCTG(+CAGTGT)GATCCGGAACGGAACCGGAAAACAGCGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605203921_4kc6 CCAGCCTCTTGAAAGTTAGGATGGGGTGGGAG(+CAGTGT)GATCCCACTTTTCTTATTGGCTGGAGGACAA NGG 10 8 17 8 24 0 100 5 10
    240605203920_eo3i GCACATTGTCCTCCAGCCAATAAGAAAAGTGG(+CAGTGT)GATCCTCCCACCCCATCCTAACTTTCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605203918_cw3t TCTTACTCCTGAGTATCCCAGGAGCTCAAAGG(+CAGTGT)GTACCGGGTGGTGTGCAGCGGGGTTAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605203915_x80o TGGCCCCTTCTAACCCCGCTGCACACCACCCG(+CAGTGT)GTACCCTTTGAGCTCCTGGGATACTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605203914_2c04 GCTTCAGTGGAGGCCGGCCGGGGGAGGGAAGG(+CAGTGT)GATCCTATATTTCCTGCTGACTCTTATTAGG NGG 10 8 17 8 24 0 100 5 10