Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203912_7lya CTGACCCTAATAAGAGTCAGCAGGAAATATAG(+CAGTGT)GATCCCTTCCCTCCCCCGGCCGGCCTCCACT NGG 10 8 17 8 24 0 100 5 10
    240605203911_pc7z TGTGCACTGCCAGGGAGGTAGGTGTTCATGGG(+CAGTGT)GTACCTCTGTACCCAGGTGCTGCCCACTCCC NGG 10 8 17 8 24 0 100 5 10
    240605203909_e96o ACACGGGGAGTGGGCAGCACCTGGGTACAGAG(+CAGTGT)GTACCCCATGAACACCTACCTCCCTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605203907_kjq2 CTTTAGTGGCACTTGCCACACAGCTCCAGGGG(+CAGTGT)GTACCATTGGCACTGCAGGCTGGGCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605203905_ychq TGCCACAGACTGCCCAGCCTGCAGTGCCAATG(+CAGTGT)GTACCCCCTGGAGCTGTGTGGCAAGTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240605203903_iavx GTATAGAGAGAGTTGTTGTCGTGGGGCACCCG(+CAGTGT)GATCCCAGCAGCCTGGTTGCCTGCCTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605203902_31d3 GACATCCTACAGGCAGGCAACCAGGCTGCTGG(+CAGTGT)GATCCGGGTGCCCCACGACAACAACTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605203859_hiea ACCGGAACTCTGCTGAGATTCCAAGGATTGGG(+CAGTGT)GTACCCCGTGTAGACTGGTGAGAGAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605203857_b2xc CTGTTCTCCTCTCTCTCACCAGTCTACACGGG(+CAGTGT)GTACCCCAATCCTTGGAATCTCAGCAGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605203856_ily4 CCCCATCTGGCCTATGCAGAATCCATTCCTTG(+CAGTGT)GTACCAGCTGGTGACTTGTAAAAGCAAACCT NGG 10 8 17 8 24 0 100 5 10