Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605204005_zqh3 AGAGTCAAGAGAGTTGTAGTGTTGTGGACATG(+CAGTGT)GTACCTAACTCAGGCCTCCAAGGAGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605204004_h86a TTGTGAGTGTTAAACGTGTGGCTGTATTAAAG(+CAGTGT)GATCCTGTCAGGGTTTCTCATTCCCAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605204002_zoy6 TGGAAGGCTTTGGGAATGAGAAACCCTGACAG(+CAGTGT)GATCCTTTAATACAGCCACACGTTTAACACT NGG 10 8 17 8 24 0 100 5 10
    240605204000_bmtk TAACACCTAGTTCTGGCCTCCTCAGGTACTGG(+CAGTGT)GTACCCACATGGTGGACATACATACATACAT NGG 10 8 17 8 24 0 100 5 10
    240605203959_hdbn CCTACATGTATGTATGTATGTCCACCATGTGG(+CAGTGT)GTACCCAGTACCTGAGGAGGCCAGAACTAGG NGG 10 8 17 8 24 0 100 5 10
    240605203956_jy18 TCTCAGCCAGCTGAGCCACTGGCTATCCTGGG(+CAGTGT)GATCCACACAGATCAAATTATGGGTTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605203955_5vj7 CCACTTCCCCAACCCATAATTTGATCTGTGTG(+CAGTGT)GATCCCCAGGATAGCCAGTGGCTCAGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605203953_qgpj GGTACACAAACGGGTAACCCTCTGTCTCTGGG(+CAGTGT)GTACCTAGAACTACTACTTAGCCTGAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605203952_mgj9 GACACCCCACTCAGGCTAAGTAGTAGTTCTAG(+CAGTGT)GTACCCCAGAGACAGAGGGTTACCCGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605203950_wxft GATCCCAGGGTTTGCCAACACAGAGGCTAGCG(+CAGTGT)GTACCCCCAAAACCCAGGGAAAGGAGGTCAT NGG 10 8 17 8 24 0 100 5 10