Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203854_gqtp GAGAGAGGTTTGCTTTTACAAGTCACCAGCTG(+CAGTGT)GTACCAAGGAATGGATTCTGCATAGGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605203852_c7i8 TTGTTGTTTTCCATTTTGAAGGGTGAGAGTTG(+CAGTGT)GTACCTAGGACAGTACTCAGCATGTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605203850_wmnh AAAGGCCAGGCACATGCTGAGTACTGTCCTAG(+CAGTGT)GTACCAACTCTCACCCTTCAAAATGGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605203849_e58c CTCCCTAGCTTACGCTCTGGGAGGGGTACTGG(+CAGTGT)GTACCCCACCCTTCACTGAGGCCTTTGGATT NGG 10 8 17 8 24 0 100 5 10
    240605203846_75eu AGACAAATCCAAAGGCCTCAGTGAAGGGTGGG(+CAGTGT)GTACCCAGTACCCCTCCCAGAGCGTAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605203844_lqow TAGTGCGATTAATACAAACGCTTTAACAAGTG(+CAGTGT)GTACCTCTGGAAGATTCAAGAGAGGAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605203842_xtp2 AAGAGTTGCCTCCTCTCTTGAATCTTCCAGAG(+CAGTGT)GTACCACTTGTTAAAGCGTTTGTATTAATCG NGG 10 8 17 8 24 0 100 5 10
    240605203841_ol7q CCCTAACTGTATGGACCATCTTCAAACTGCTG(+CAGTGT)GATCCCCTGTATATGTTGAATGATGCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605203839_ebok AGACCACCAGGCATCATTCAACATATACAGGG(+CAGTGT)GATCCAGCAGTTTGAAGATGGTCCATACAGT NGG 10 8 17 8 24 0 100 5 10
    240605203837_n9v6 CTGCAGCCAGGGCTTCCCCCATCCCTGGGGAG(+CAGTGT)GATCCAGCACCAGCCTGAGAAGGGAGGCAAC NGG 10 8 17 8 24 0 100 5 10