Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203801_gc3o CGCGCCCAGTCCAGGATCTGCCTCCTATCAGG(+CAGTGT)GATCCCAACTCTGAATTTCCTGCCACTAACA NGG 10 8 17 8 24 0 100 5 10
    240605203759_8pry TGCCAGCTCAGGGTCCCCTCCACTGGTTCTTG(+CAGTGT)GTACCTACCACCCTCCTTTAAAGACCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605203758_vmtl GGTGCCTCTGGGTCTTTAAAGGAGGGTGGTAG(+CAGTGT)GTACCAAGAACCAGTGGAGGGGACCCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605203756_ewrs TGTCTGTTCCTGCTTTTCTGTCCTGGGCTGGG(+CAGTGT)GATCCAGGCTCAGCACTTTCTCCCCGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605203754_gh9o ATAGTGGGGACGGGGAGAAAGTGCTGAGCCTG(+CAGTGT)GATCCCCAGCCCAGGACAGAAAAGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605203753_jws8 TTAAGAAATTAATATAGAAGGAGATGAAAGTG(+CAGTGT)GATCCTGAAAGGGTGACAGGAGGAATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605203751_ief7 TAACATTTCCATTCCTCCTGTCACCCTTTCAG(+CAGTGT)GATCCACTTTCATCTCCTTCTATATTAATTT NGG 10 8 17 8 24 0 100 5 10
    240605203750_jhi4 AACCTGTTCCTGGAGGTGGAACTCTTCATCAG(+CAGTGT)GATCCCCAAAGGCGTGCTGGAATTAGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605203748_w1xt GTTCAGTGGACTAATTCCAGCACGCCTTTGGG(+CAGTGT)GATCCTGATGAAGAGTTCCACCTCCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605203747_ujd1 TTCTATAACCAATACCTTGACCCTCTACAGTG(+CAGTGT)GATCCAGAAATGCTGAGAACCCACAGGCTGA NGG 10 8 17 8 24 0 100 5 10