Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605203716_6sab GGCAGCTGCTGTCTGTTCTGTGTCCATAGTAG(+CAGTGT)GATCCTGCCCAAAGAGGAGCGGCCTCCCCGC NGG 10 8 17 8 24 0 100 5 10
    240605203714_kwj5 ATTAGAAAGGGTGGCAGGTCAAGTCGAGGAAG(+CAGTGT)GTACCATCAGCCCAAGCAAAAAGCCCGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605203712_r8as AGCCAAACCCGGGCTTTTTGCTTGGGCTGATG(+CAGTGT)GTACCTTCCTCGACTTGACCTGCCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605203711_aeae AATACATTTTATGCCGGGGGTAGGGGGACAGG(+CAGTGT)GATCCGTGCTATTGTGGAGCAGCACTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240605203709_hbgy TTCAGGTTACAGTGCTGCTCCACAATAGCACG(+CAGTGT)GATCCCTGTCCCCCTACCCCCGGCATAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605203708_27s1 AACCCCTCAAAGCAGAGAGGTCTGGGGAACAG(+CAGTGT)GTACCCCCTAGCTCCGGATACCACTGCGTCC NGG 10 8 17 8 24 0 100 5 10
    240605203706_59fr GGGTGGGACGCAGTGGTATCCGGAGCTAGGGG(+CAGTGT)GTACCTGTTCCCCAGACCTCTCTGCTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605203704_forz AGCTTTGCCCTTACTGATCTTACTTTCCCGAG(+CAGTGT)GTACCTACGATGCTCTCTGATGCCAGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605203702_cych CCAAGATCTGCTGGCATCAGAGAGCATCGTAG(+CAGTGT)GTACCTCGGGAAAGTAAGATCAGTAAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605203700_5dqy TGGTCACCACAGGAAACAAGAAAAGACTAGGG(+CAGTGT)GTACCGAGGACATTTTCTAGGAACAGAAGAT NGG 10 8 17 8 24 0 100 5 10