Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171151_0tz9 GCCTTAGTCCTTCCCTCTTTAATCCCCTATGG(+AGGACA)GATCCTTCTCTGTGTTCCTGTGTGTTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240613171150_n0wo ATGAGAAGCAAACACACAGGAACACAGAGAAG(+AGGACA)GATCCCATAGGGGATTAAAGAGGGAAGGACT NGG 10 8 17 8 24 0 100 5 10
    240613171149_kq5d ACACTGAGGCTTCATTCTGTTCTTTAGCTTTG(+AGGACA)GTACCTTTAATCCAGAGTCAATGGGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613171147_8nci CTATATCTTTTCCCATTGACTCTGGATTAAAG(+AGGACA)GTACCAAAGCTAAAGAACAGAATGAAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613171146_l875 TGCACATGCCCTTTTCTGTCCAGTTTAGGTTG(+AGGACA)GATCCTTAGTACCTAGACACACTCTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613171145_nfrt CACGTAAGAGGAGAGTGTGTCTAGGTACTAAG(+AGGACA)GATCCAACCTAAACTGGACAGAAAAGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613171143_jeg3 TGTGTCCCCAATCCCATCCATCTGTCTGAAAG(+AGGACA)GATCCCAGCATCCCTAGGCTTGGCATCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613171142_5txt CCTTCCTCAGATGCCAAGCCTAGGGATGCTGG(+AGGACA)GATCCTTTCAGACAGATGGATGGGATTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613171140_bvty AAGTTTAAATAATCTAAGTTTCTTTGCAGCTG(+AGGACA)GATCCAACTGCTAGAAGGCAATTCTTAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613171139_0gki AGATTGCTCTAAGAATTGCCTTCTAGCAGTTG(+AGGACA)GATCCAGCTGCAAAGAAACTTAGATTATTTA NGG 10 8 17 8 24 0 100 5 10