Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171104_t81f TGGTTGGCTTGGGTTTGCTTGGTCTTGTGCGG(+AGGACA)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613171102_apl9 TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+AGGACA)GTACCCGCACAAGACCAAGCAAACCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613171100_2qg7 CTTTCTTTCTTTTTTTTTTCTTTTTAAAATGG(+AGGACA)GATCCACTGTGTTGAAGACTCTTGATATCAT NGG 10 8 17 8 24 0 100 5 10
    240613171059_s3ku AGCACATGATATCAAGAGTCTTCAACACAGTG(+AGGACA)GATCCCATTTTAAAAAGAAAAAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613171057_sgso TCTCAAGGTCTTGGTCAGGACCTCACTACCAG(+AGGACA)GTACCCGCACCCCCAACCTTTCAATAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240613171055_f10f GGGTTCTTGCTATTGAAAGGTTGGGGGTGCGG(+AGGACA)GTACCTGGTAGTGAGGTCCTGACCAAGACCT NGG 10 8 17 8 24 0 100 5 10
    240613171054_pgkx TCCTATTTCTCCTTGAATATTAACTTGGAAAG(+AGGACA)GTACCCAGTAAGCCAAGCACTGCCCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240613171053_6nnm CTGGTCTGTCTGGGCAGTGCTTGGCTTACTGG(+AGGACA)GTACCTTTCCAAGTTAATATTCAAGGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613171051_oorf TTCATTGACCAACCAGTCTTAAGAATGAAGTG(+AGGACA)GTACCCAGGCTCATTGAGAGATCCTGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613171049_dwfg TTTTTTGAGACAGGATCTCTCAATGAGCCTGG(+AGGACA)GTACCACTTCATTCTTAAGACTGGTTGGTCA NGG 10 8 17 8 24 0 100 5 10