Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171032_fnme TGGAAGCAAGGATGAACGCTTTCCTAGGTCTG(+AGGACA)GTACCGTTTCCTCTCAATGGCCTCACCATAC NGG 10 8 17 8 24 0 100 5 10
    240613171031_3i7g ACTGAGTATGGTGAGGCCATTGAGAGGAAACG(+AGGACA)GTACCAGACCTAGGAAAGCGTTCATCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240613171030_ddvv CACCCCTGGATGCTGTCTCCCAGCCAAAACTG(+AGGACA)GTACCTGTACTTACAGAAATCAAATTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613171028_xkix AATTGCCTGCAATTTGATTTCTGTAAGTACAG(+AGGACA)GTACCAGTTTTGGCTGGGAGACAGCATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613171025_c46r ATATCTATATTTGTGTACCATGTGCGCCCCTG(+AGGACA)GTACCCAAGAAGATCAGAAGAGGGTGTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613171024_jb16 CAAGATCTGACACCCTCTTCTGATCTTCTTGG(+AGGACA)GTACCAGGGGCGCACATGGTACACAAATATA NGG 10 8 17 8 24 0 100 5 10
    240613171022_okur TGGGCAAGGAACAGGTGAAGGCTAGACTTTGG(+AGGACA)GTACCAAACAGTCCTCGATCTGCAGATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613171021_tn4g ACGCTCAGCATCTGCAGATCGAGGACTGTTTG(+AGGACA)GTACCCAAAGTCTAGCCTTCACCTGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613171019_7w5p TTTCTTGATCTCAGAATAAATATTTCAAAGGG(+AGGACA)GATCCAAAAATGCTGGACTTTTAAATATACT NGG 10 8 17 8 24 0 100 5 10
    240613171017_8y6e CTAAAAGTATATTTAAAAGTCCAGCATTTTTG(+AGGACA)GATCCCCTTTGAAATATTTATTCTGAGATCA NGG 10 8 17 8 24 0 100 5 10