Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171016_hq6n ACAATGTTTGGAATGTTATATTGGGACAAATG(+AGGACA)GTACCCAAGTGATCAGACATATAAAGTAGAC NGG 10 8 17 8 24 0 100 5 10
    240613171015_7pid CATTTGTCTACTTTATATGTCTGATCACTTGG(+AGGACA)GTACCATTTGTCCCAATATAACATTCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613171013_0sqk AACCTTTCTCAGACCCACAAGTCCCAAACCTG(+AGGACA)GATCCCAGTTTTATCCCATAATTTCTTCAAT NGG 10 8 17 8 24 0 100 5 10
    240613171011_0qib GTTGTATTGAAGAAATTATGGGATAAAACTGG(+AGGACA)GATCCAGGTTTGGGACTTGTGGGTCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613171010_9rzk CTACGTTCATCCAGGCAGCAACAGAAGCTACG(+AGGACA)GATCCTTAACATATCAATGAGCTCTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240613171009_ro6a TATTTAAGTTCAGAGCTCATTGATATGTTAAG(+AGGACA)GATCCGTAGCTTCTGTTGCTGCCTGGATGAA NGG 10 8 17 8 24 0 100 5 10
    240613171008_nuuy GGTGGAGTGGATTGATTGGAGGCTGAGAAAAG(+AGGACA)GTACCAAAGCACAGAGGTTGAAACAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240613171006_3itt CTAGAATTTATTGTTTCAACCTCTGTGCTTTG(+AGGACA)GTACCTTTTCTCAGCCTCCAATCAATCCACT NGG 10 8 17 8 24 0 100 5 10
    240613171005_meqk AAAAACCTTGATAATAGGCTAGGTTGTTGGAG(+AGGACA)GATCCCATGGGGCCCCTTCAGCCAACAACTA NGG 10 8 17 8 24 0 100 5 10
    240613171002_3k24 TAATCTAGTTGTTGGCTGAAGGGGCCCCATGG(+AGGACA)GATCCTCCAACAACCTAGCCTATTATCAAGG NGG 10 8 17 8 24 0 100 5 10