Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170913_pj0i GCTTGTAAACTATGAGGCACTACAGTGTCCAG(+AGGACA)GATCCCCACGTCGGCCAAGCAGCTCTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613170910_lw02 GACCTGCTGCAGAGCTGCTTGGCCGACGTGGG(+AGGACA)GATCCTGGACACTGTAGTGCCTCATAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240613170909_rs7h GAGCTATGGCCCTGACCTGTGGATAACAAACG(+AGGACA)GATCCCTCGTGATGTAGCCTGGGCAGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613170908_r07g TGTCCACTAACTGCCCAGGCTACATCACGAGG(+AGGACA)GATCCGTTTGTTATCCACAGGTCAGGGCCAT NGG 10 8 17 8 24 0 100 5 10
    240613170906_r0j2 AAAATAAGTACAACATCTGATGTCCAAACAGG(+AGGACA)GATCCTTCTGGGTGTGGCTTTACCCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240613170904_rjsr TGCAGTTGCATGGGTAAAGCCACACCCAGAAG(+AGGACA)GATCCCTGTTTGGACATCAGATGTTGTACTT NGG 10 8 17 8 24 0 100 5 10
    240613170903_6a4x AAGGGTAGCAACAGCTTGTCTTCTGATTGATG(+AGGACA)GTACCATCCCCCAAGAAGGGATCCTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613170902_bbuw TTCTTCCTGAGAGGATCCCTTCTTGGGGGATG(+AGGACA)GTACCATCAATCAGAAGACAAGCTGTTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613170900_cw3h GGAGCTATGGAAGCATTGGTCTCTACACAGCG(+AGGACA)GTACCCACAGGACCCACACAATGAGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240613170859_w355 AAATGCTAACCCTCATTGTGTGGGTCCTGTGG(+AGGACA)GTACCGCTGTGTAGAGACCAATGCTTCCATA NGG 10 8 17 8 24 0 100 5 10