Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170825_dbg1 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613170823_durg TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613170822_8lcg GTGTCCTGGATTACCTGGATGTTTTGAGTTAG(+AGGACA)GATCCTTTTGCATTTTGTATTTTCTTTGACT NGG 10 8 17 8 24 0 100 5 10
    240613170821_pukk ACAACAGTCAAAGAAAATACAAAATGCAAAAG(+AGGACA)GATCCTAACTCAAAACATCCAGGTAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613170819_eptk CCCCTTAACATGCCCTCCCTCCAACCCCTGAG(+AGGACA)GATCCAGAAATGCCTGCTTTAGAATAGACAA NGG 10 8 17 8 24 0 100 5 10
    240613170818_egzc GCCCTTTGTCTATTCTAAAGCAGGCATTTCTG(+AGGACA)GATCCTCAGGGGTTGGAGGGAGGGCATGTTA NGG 10 8 17 8 24 0 100 5 10
    240613170816_esgm GACAAAGGATAACAGAATGGATTAAAAAACAG(+AGGACA)GATCCATCATTCTGCTGTATACAGCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240613170815_n7v4 GAGATGTATTTGCTGTATACAGCAGAATGATG(+AGGACA)GATCCTGTTTTTTAATCCATTCTGTTATCCT NGG 10 8 17 8 24 0 100 5 10
    240613170814_gg5d GCTGACTTCAGACACACCAGAAGAAATCATCG(+AGGACA)GATCCCATTACGAATGTTTGGGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613170813_16z8 ACCACATGGTGGCTCCCAAACATTCGTAATGG(+AGGACA)GATCCGATGATTTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10