Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170755_66yf TTGGATTAAAAGCCTGTTTATCTCCGTATTTG(+AGGACA)GATCCCTGGATTCTATAGCCTTGCCAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240613170752_wi8k GTTGAGTCTTTGGCAAGGCTATAGAATCCAGG(+AGGACA)GATCCAAATACGGAGATAAACAGGCTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613170750_bk7a TCAAAGACCTCAAGAACTGCCTATGGACCCAG(+AGGACA)GTACCTTCCCAATGCATGAGCTACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240613170748_974l GAAGGTGGGTTTGTAGCTCATGCATTGGGAAG(+AGGACA)GTACCTGGGTCCATAGGCAGTTCTTGAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613170746_fi0k CACCAAGCCTGATATCTGTGTTTGAACCCTAG(+AGGACA)GATCCATATGGTAGGAGAGAACTAACCCACA NGG 10 8 17 8 24 0 100 5 10
    240613170744_mvkh CAACTTGTGGGTTAGTTCTCTCCTACCATATG(+AGGACA)GATCCTAGGGTTCAAACACAGATATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613170742_s1di TGGGTTTGGACTCCTTGTCTACATCTGTCCCG(+AGGACA)GATCCTCAACGCTCTCCAAAGGACAGCCTAC NGG 10 8 17 8 24 0 100 5 10
    240613170741_goct CGGTGGTAGGCTGTCCTTTGGAGAGCGTTGAG(+AGGACA)GATCCGGGACAGATGTAGACAAGGAGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613170739_vl4x TATATTTAAAAAACAAAAACAGTCTTGCCATG(+AGGACA)GATCCAGTGTTAACACTGTCATTTTGTAACA NGG 10 8 17 8 24 0 100 5 10
    240613170738_xz1p AAGGCTGTTACAAAATGACAGTGTTAACACTG(+AGGACA)GATCCATGGCAAGACTGTTTTTGTTTTTTAA NGG 10 8 17 8 24 0 100 5 10