Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170722_lpoq TAGCACAGGGTCCAGGCAACCTCAGCCTATGG(+AGGACA)GATCCTTAGGCACTGTGTCACAGGAACGTCC NGG 10 8 17 8 24 0 100 5 10
    240613170720_n3ci AGCTAGGACGTTCCTGTGACACAGTGCCTAAG(+AGGACA)GATCCCATAGGCTGAGGTTGCCTGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240613170719_leju GTTTCAAGATTTTCTAACACAAATTTACATTG(+AGGACA)GTACCAAAATGGGCAAGAAAATGAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240613170718_vvrg GAGCCTGTGCCTTCATTTTCTTGCCCATTTTG(+AGGACA)GTACCAATGTAAATTTGTGTTAGAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240613170716_hles CCAAAAAATACAGCAAAGACAGGAACGTCCAG(+AGGACA)GATCCGGGTCTGTTAATAAAGCCACCTCGAG NGG 10 8 17 8 24 0 100 5 10
    240613170713_cfkf CTAGTCTCGAGGTGGCTTTATTAACAGACCCG(+AGGACA)GATCCTGGACGTTCCTGTCTTTGCTGTATTT NGG 10 8 17 8 24 0 100 5 10
    240613170712_tg3i ATCAGGCTGGGGAGGTTAGTGCACAATCAGTG(+AGGACA)GATCCATGCCCCGCCTCCAAGCTCTGCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613170711_vg2s GTGGGGTTTGCAGAGCTTGGAGGCGGGGCATG(+AGGACA)GATCCACTGATTGTGCACTAACCTCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613170709_xdxs CAGAAGTCAGAGTCATCAGTCATTTGGCTAAG(+AGGACA)GTACCGCTGAGACACAATATCTCTGTAACTT NGG 10 8 17 8 24 0 100 5 10
    240613170708_hbh6 AAAGAAAGTTACAGAGATATTGTGTCTCAGCG(+AGGACA)GTACCTTAGCCAAATGACTGATGACTCTGAC NGG 10 8 17 8 24 0 100 5 10