Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170653_3whh AATAAAGTCAGATCATTACAATCTTAACTGTG(+AGGACA)GTACCCTTTTTTGATCAAAAAAACCTAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613170651_rny9 AACAGTAACTAGGTTTTTTTGATCAAAAAAGG(+AGGACA)GTACCACAGTTAAGATTGTAATGATCTGACT NGG 10 8 17 8 24 0 100 5 10
    240613170649_d5m0 TTCCGACTTTATCATTTGAGGAGAGAACAAGG(+AGGACA)GTACCGTCTTGTAATTTTGCCTTTCTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240613170648_br4a AATGTTACCAAGAAAGGCAAAATTACAAGACG(+AGGACA)GTACCCTTGTTCTCTCCTCAAATGATAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613170647_1920 ACATTCAGGTCTGAATTCATATCTCAAGAAAG(+AGGACA)GATCCTCCCTGTCTCTTTTAGTGTCTCATCA NGG 10 8 17 8 24 0 100 5 10
    240613170645_hhbk AGCTCTGATGAGACACTAAAAGAGACAGGGAG(+AGGACA)GATCCTTTCTTGAGATATGAATTCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240613170644_oduo CAGCTAGAGGAATTGTTCCAAGGTTAATAGTG(+AGGACA)GATCCCCAGATTAGATGTTAAAGAATAGATT NGG 10 8 17 8 24 0 100 5 10
    240613170642_pdo0 CATCTAATCTATTCTTTAACATCTAATCTGGG(+AGGACA)GATCCACTATTAACCTTGGAACAATTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613170641_7f57 CTTAGTCTGGGGTAAAGAGGCTGGTGAAGTAG(+AGGACA)GTACCTCCAATTGCAGTGTGGTCACTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613170639_m2f2 ATGGTATTAAAGTGACCACACTGCAATTGGAG(+AGGACA)GTACCTACTTCACCAGCCTCTTTACCCCAGA NGG 10 8 17 8 24 0 100 5 10