Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170707_v45p GGAAAAAACTGAAACACTAAGTGTAGGAAGAG(+AGGACA)GATCCTAGAAAACTAAACGTATTGACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613170705_90sf TTATTTCAAAGTCAATACGTTTAGTTTTCTAG(+AGGACA)GATCCTCTTCCTACACTTAGTGTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613170704_q9v7 CTTCACACAACATCTGCATTCACATATTTTAG(+AGGACA)GATCCTTTGTCTCTCTTCCTGTACACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613170703_aw7p AGATGAAAAAGTGTACAGGAAGAGAGACAAAG(+AGGACA)GATCCTAAAATATGTGAATGCAGATGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613170702_e0le TGGTCCAAAAGTCAATGGCTGCAGTCCGTGTG(+AGGACA)GATCCAATGGTAGAGACTTTTTTCTAGTACA NGG 10 8 17 8 24 0 100 5 10
    240613170700_01e2 AAAAGTGTACTAGAAAAAAGTCTCTACCATTG(+AGGACA)GATCCACACGGACTGCAGCCATTGACTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613170659_kq0f TGCTCCTACAAGGCTCACAGAAGAAGAGCATG(+AGGACA)GATCCCCTTCAGCAAGAATGCTTAAGACTCG NGG 10 8 17 8 24 0 100 5 10
    240613170657_tf7q TGACACGAGTCTTAAGCATTCTTGCTGAAGGG(+AGGACA)GATCCATGCTCTTCTTCTGTGAGCCTTGTAG NGG 10 8 17 8 24 0 100 5 10
    240613170655_j8m0 AAAGGAAAGAGGAAAAAAAAAGGTCAAAAAAG(+AGGACA)GTACCAAAAGTCACACACAAAAAACACAAGA NGG 10 8 17 8 24 0 100 5 10
    240613170654_ze6i AATACTCTTGTGTTTTTTGTGTGTGACTTTTG(+AGGACA)GTACCTTTTTTGACCTTTTTTTTTCCTCTTT NGG 10 8 17 8 24 0 100 5 10