Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170638_o3s3 TGAAATTGTCAATTTCAGAGCATGAAGTGAAG(+AGGACA)GTACCGACTCAAGAAAAAGATTTTTACTGCA NGG 10 8 17 8 24 0 100 5 10
    240613170637_8i5f ACTGATGCAGTAAAAATCTTTTTCTTGAGTCG(+AGGACA)GTACCTTCACTTCATGCTCTGAAATTGACAA NGG 10 8 17 8 24 0 100 5 10
    240613170635_pml3 GGTTATAGTTTCAGTGTAGCTCTGGGAACAGG(+AGGACA)GATCCCTGGACCACTAATACTTTCTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240613170633_c5cv TAGATAGATCTAGAAAGTATTAGTGGTCCAGG(+AGGACA)GATCCCTGTTCCCAGAGCTACACTGAAACTA NGG 10 8 17 8 24 0 100 5 10
    240613170632_fkwn AATTTTCCATTCCTGCATTAGTCTAGTGTATG(+AGGACA)GATCCACGTGCTCTCTTTTAGTATTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240613170630_wbiy ACTTATAGGATAATACTAAAAGAGAGCACGTG(+AGGACA)GATCCATACACTAGACTAATGCAGGAATGGA NGG 10 8 17 8 24 0 100 5 10
    240613170629_nqba TAAATTCAATATAAATTGTGATCTCTTGCTGG(+AGGACA)GATCCCAGGCCCATTGTGTTTCCAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613170627_zwuh TCCCTACAGCCCTGGAAACACAATGGGCCTGG(+AGGACA)GATCCCAGCAAGAGATCACAATTTATATTGA NGG 10 8 17 8 24 0 100 5 10
    240613170625_ysu8 TAGGTAACAAATTTATCAATCAACTATGTTTG(+AGGACA)GATCCAGGGAATCTCAAGTTATTCAATTCAT NGG 10 8 17 8 24 0 100 5 10
    240613170623_82l8 AGAGAATGAATTGAATAACTTGAGATTCCCTG(+AGGACA)GATCCAAACATAGTTGATTGATAAATTTGTT NGG 10 8 17 8 24 0 100 5 10