Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170737_h7oa AGACCCCACACTTTTTGTAAGACATCAATCAG(+AGGACA)GATCCCCATATTAATTATAAGCATCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240613170735_a5s3 CAACATTCAGTGATGCTTATAATTAATATGGG(+AGGACA)GATCCTGATTGATGTCTTACAAAAAGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240613170734_i618 TGACACTGAAGGAATAGGCAGAGGTTTTGAAG(+AGGACA)GTACCTCATAGCTACCACAATGATAATAACA NGG 10 8 17 8 24 0 100 5 10
    240613170732_6gfh GTGGTTGTTATTATCATTGTGGTAGCTATGAG(+AGGACA)GTACCTTCAAAACCTCTGCCTATTCCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613170731_ibcn CACACATCTGTGCACTGACTTTGCCATTGAGG(+AGGACA)GATCCTCTGGGTTGGGTCTTTAAGACGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613170729_3nbb GTTTCCCTTCGTCTTAAAGACCCAACCCAGAG(+AGGACA)GATCCCTCAATGGCAAAGTCAGTGCACAGAT NGG 10 8 17 8 24 0 100 5 10
    240613170727_1i24 GCCCTTCCCACCTTTGCCTGTCTACAGTAGTG(+AGGACA)GATCCATTTCCTCCCGAGGTTGCCCATCAAT NGG 10 8 17 8 24 0 100 5 10
    240613170726_ide6 ATCCAATTGATGGGCAACCTCGGGAGGAAATG(+AGGACA)GATCCACTACTGTAGACAGGCAAAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613170725_57sy ATACTCCCAGAATGAGCAGAGGGTCCAAAGTG(+AGGACA)GATCCCTGAGCCATTTTGAATAATTTTATCA NGG 10 8 17 8 24 0 100 5 10
    240613170723_iynn AGTGGTGATAAAATTATTCAAAATGGCTCAGG(+AGGACA)GATCCACTTTGGACCCTCTGCTCATTCTGGG NGG 10 8 17 8 24 0 100 5 10