Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170930_ezlw CAACCCACAAGCAGGTGTGTGAACCAGAATGG(+AGGACA)GATCCGCTGATGGCTGGCATGCCTTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613170929_8psw TAGCCTCTCCCAAGGCATGCCAGCCATCAGCG(+AGGACA)GATCCCATTCTGGTTCACACACCTGCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613170927_15d9 GTCATAACCCCCACCCCACCCCACCCCCGGTG(+AGGACA)GTACCTTAAAGAGATTAAATGCAAACATTCC NGG 10 8 17 8 24 0 100 5 10
    240613170926_f24i AGCAAGGAATGTTTGCATTTAATCTCTTTAAG(+AGGACA)GTACCACCGGGGGTGGGGTGGGGTGGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613170922_mt2n ACATCTAGAATGTTCATTTTTCCTGCCCCCAG(+AGGACA)GTACCCTAGTCAGGTGTGGGCTAGCCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613170921_omqp TGAATCACCTGGCTAGCCCACACCTGACTAGG(+AGGACA)GTACCTGGGGGCAGGAAAAATGAACATTCTA NGG 10 8 17 8 24 0 100 5 10
    240613170919_zhks CCTCGCTTAGGATGGAGGAGGGGATGCTGTGG(+AGGACA)GTACCACCCCACCCCAGCAGTACTCCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613170917_q7i0 AAGTCCCCAGGGAGTACTGCTGGGGTGGGGTG(+AGGACA)GTACCCACAGCATCCCCTCCTCCATCCTAAG NGG 10 8 17 8 24 0 100 5 10
    240613170916_g6mb GCTCTCAGAAAGGTGCCCCTGCTAGCCTTTGG(+AGGACA)GATCCTCTTGGCTACGGCACCTACTCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240613170914_5tga GTGGGTTGGAGAGTAGGTGCCGTAGCCAAGAG(+AGGACA)GATCCCAAAGGCTAGCAGGGGCACCTTTCTG NGG 10 8 17 8 24 0 100 5 10