Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170945_4r7h TCTTATGTTAAAAGGTTGGTTTGTACAAAACG(+AGGACA)GTACCTTCTTTCCATTGACTCTTGGATGCTC NGG 10 8 17 8 24 0 100 5 10
    240613170943_rmqp GCAAAGAGCATCCAAGAGTCAATGGAAAGAAG(+AGGACA)GTACCGTTTTGTACAAACCAACCTTTTAACA NGG 10 8 17 8 24 0 100 5 10
    240613170942_v6ax CCATCTTTCTGTACACTAGATTTCTTTCTTTG(+AGGACA)GATCCTTAAAGATTTCTTTCCAGTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613170941_iqvm TAAAACCTTTACACTGGAAAGAAATCTTTAAG(+AGGACA)GATCCAAAGAAAGAAATCTAGTGTACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613170939_m7a2 CCCATCCACCTGGCATTTATTTGGCTTCTAGG(+AGGACA)GATCCAAACTCCGGCTCTCTTCACGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240613170938_kf37 TACTTGCCATGCGTGAAGAGAGCCGGAGTTTG(+AGGACA)GATCCCTAGAAGCCAAATAAATGCCAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613170936_dimj GTGTGGAGCTTGGCAGCCTCTACCTGGAGTGG(+AGGACA)GTACCATCTGCCTGACGTTGCAACTGATAGC NGG 10 8 17 8 24 0 100 5 10
    240613170935_bjhn CCGTGGCTATCAGTTGCAACGTCAGGCAGATG(+AGGACA)GTACCCACTCCAGGTAGAGGCTGCCAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613170934_2yxm GTGGTTAGGGTGTATAAAACCTGAACTTGCAG(+AGGACA)GATCCGGTGAAGCCTTTATTTAGCATGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613170932_xv4t GTAGCCACACATGCTAAATAAAGGCTTCACCG(+AGGACA)GATCCTGCAAGTTCAGGTTTTATACACCCTA NGG 10 8 17 8 24 0 100 5 10