Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171048_q3qu ATATGGCTATAAATATAAATTTTGTATTACTG(+AGGACA)GTACCAAAAACACTTTTTTTTTTTTTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613171047_biz7 CCCTGCTGGAAAAAAAAAAAAAAGTGTTTTTG(+AGGACA)GTACCAGTAATACAAAATTTATATTTATAGC NGG 10 8 17 8 24 0 100 5 10
    240613171045_4bft CTAGGTGTTCAGGGACCTCCAAAAGCTCCAAG(+AGGACA)GATCCACCTCTTGCTACATCTTCAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613171043_ydok CTCTATTCCTACTGAAGATGTAGCAAGAGGTG(+AGGACA)GATCCTTGGAGCTTTTGGAGGTCCCTGAACA NGG 10 8 17 8 24 0 100 5 10
    240613171041_kg5e TTGAGCGAGCGAGAGATTATTCTAATGTCCTG(+AGGACA)GTACCCGGCTGGCTTCTGGGAGGACTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613171039_xt6c TCGAAAAGCCAGTCCTCCCAGAAGCCAGCCGG(+AGGACA)GTACCAGGACATTAGAATAATCTCTCGCTCG NGG 10 8 17 8 24 0 100 5 10
    240613171038_yuvd AAGCTTATTGGCCAAACCCTCAGGGCCTCTAG(+AGGACA)GTACCTCATTAGCATGGAGAACTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613171036_7yr4 ATCATCAGAACAGAGTTCTCCATGCTAATGAG(+AGGACA)GTACCTAGAGGCCCTGAGGGTTTGGCCAATA NGG 10 8 17 8 24 0 100 5 10
    240613171035_2klx TATGTGGGTGTGAGGTGGTCAGGCCATTTGTG(+AGGACA)GTACCCGTAGTGTTTGGTGCTGGCCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240613171033_l9c2 ATAGTGGCTAGGGCCAGCACCAAACACTACGG(+AGGACA)GTACCACAAATGGCCTGACCACCTCACACCC NGG 10 8 17 8 24 0 100 5 10