Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171120_jz62 GAAATGTTTAAATTAACTGTGTTGTACAAATG(+AGGACA)GTACCCAACACAAAACCTTTTTTAAATTAGT NGG 10 8 17 8 24 0 100 5 10
    240613171118_2uyq GTCTTACTAATTTAAAAAAGGTTTTGTGTTGG(+AGGACA)GTACCATTTGTACAACACAGTTAATTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240613171117_kmrb TGGTGGGCTCAGGTTTGCTTGGTCTTGTGTGG(+AGGACA)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613171115_c70g TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+AGGACA)GTACCCACACAAGACCAAGCAAACCTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613171114_dmkk TGTTTTAGGCTGGTGGGAATAAAGATGCTATG(+AGGACA)GATCCTTTCTTTTCTTTTTTTTTTTAAGTAC NGG 10 8 17 8 24 0 100 5 10
    240613171113_r9th CAATAGTACTTAAAAAAAAAAAGAAAAGAAAG(+AGGACA)GATCCATAGCATCTTTATTCCCACCAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240613171111_n0bx TGGTGGGCTCGGGTTTGCTTGGTCTTGTGAGG(+AGGACA)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613171110_yfbc TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+AGGACA)GTACCCTCACAAGACCAAGCAAACCCGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613171107_4g65 TGGTGGGCTCGGGCTTGCTTGGTCTTGTGCGG(+AGGACA)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613171106_4bqf TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+AGGACA)GTACCCGCACAAGACCAAGCAAGCCCGAGCC NGG 10 8 17 8 24 0 100 5 10