Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171000_141m CAGTCAACCTCAGGAGGTCGGATGTTGAGGGG(+AGGACA)GATCCTCCATAATGTACCAGAGACCTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613170959_9b34 CTCACTTCCCAGGTCTCTGGTACATTATGGAG(+AGGACA)GATCCCCCTCAACATCCGACCTCCTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613170957_1co9 AAATCAGTAATGTTATCTAGCTAAAAGAATAG(+AGGACA)GTACCCTGCATTATATTATGTAAGATTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613170956_mem1 TTTGTTTTTAATCTTACATAATATAATGCAGG(+AGGACA)GTACCTATTCTTTTAGCTAGATAACATTACT NGG 10 8 17 8 24 0 100 5 10
    240613170954_b91r TCCTCCAACATTTGTCTAAAAAGTATCCAGTG(+AGGACA)GTACCTGGAGGGCTGAACAGTTGTACTATAA NGG 10 8 17 8 24 0 100 5 10
    240613170952_wy14 TCAAGTTATAGTACAACTGTTCAGCCCTCCAG(+AGGACA)GTACCACTGGATACTTTTTAGACAAATGTTG NGG 10 8 17 8 24 0 100 5 10
    240613170950_3v0g CTGGACTGTTTAGAAGAACATACTAAACACGG(+AGGACA)GTACCAAAGGTGACTGCTTTCTTTAGTAACT NGG 10 8 17 8 24 0 100 5 10
    240613170948_yczh AACTGAGTTACTAAAGAAAGCAGTCACCTTTG(+AGGACA)GTACCCGTGTTTAGTATGTTCTTCTAAACAG NGG 10 8 17 8 24 0 100 5 10
    240613170947_tgrg ATCCTACTCTGAAAAAAGAAAACAACACTCTG(+AGGACA)GTACCTCTAGTTCAAAGGACTGACAAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240613170946_88lm CACGTATGCCTTGTCAGTCCTTTGAACTAGAG(+AGGACA)GTACCAGAGTGTTGTTTTCTTTTTTCAGAGT NGG 10 8 17 8 24 0 100 5 10