Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172436_3e0s AAAAAACTCTTCGTGCTAGGCTGGGCTGGCAG(+CAGTGT)GTACCATGTACCAGTCCCTTTCCTACTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605172434_d3sh GCTGAGAATCACAGATTTAGCAGCTCCTAGGG(+CAGTGT)GTACCCACCACATTGAGCTATGCTGAATTAA NGG 10 8 17 8 24 0 100 5 10
    240605172432_y5kl AAATATTAATTCAGCATAGCTCAATGTGGTGG(+CAGTGT)GTACCCCTAGGAGCTGCTAAATCTGTGATTC NGG 10 8 17 8 24 0 100 5 10
    240605172431_9dhj TCCCCAGGCCCTTGATAACTTTTCCCCTTCAG(+CAGTGT)GATCCCACTCATAGTAGATTTATTTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605172430_6dzd AATCCTACTTAAAATAAATCTACTATGAGTGG(+CAGTGT)GATCCTGAAGGGGAAAAGTTATCAAGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605172428_tcbj CTTGTACTTCTGTTACTCTAGAACCACTCCTG(+CAGTGT)GTACCAACTTCTGTATTAGTCAGGGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605172427_975l ACTCTAGAGAACCCTGACTAATACAGAAGTTG(+CAGTGT)GTACCAGGAGTGGTTCTAGAGTAACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605172425_u79p TTCTTTAAAGTTCTGGAGAATGGGTGAAGCTG(+CAGTGT)GTACCTTGCAGAGAAGGGACACAAGGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605172424_v7h8 GTGGCATCTTCCTTGTGTCCCTTCTCTGCAAG(+CAGTGT)GTACCAGCTTCACCCATTCTCCAGAACTTTA NGG 10 8 17 8 24 0 100 5 10
    240605172423_ylt5 AAAAACTTCAGAGTTGCAGACAGGACAGTCAG(+CAGTGT)GTACCCCATGGGAGTTCCTAGGGCCTGCGTA NGG 10 8 17 8 24 0 100 5 10