Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172407_iu57 TGCTTTTATCTCACAAACCCCCCACAGGCAGG(+CAGTGT)GATCCACAATGACTCCATATCCTTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605172405_70xj ACATGCTGGGAGAAAATTAACACTAAAATCTG(+CAGTGT)GTACCAGCCAGAGGAAGGAACTTGACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605172404_6fwe CTTATTTTGAGTCAAGTTCCTTCCTCTGGCTG(+CAGTGT)GTACCAGATTTTAGTGTTAATTTTCTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605172402_zucr GGTGTCAGCGTTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCCAGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605172400_4pen GGATGGACCATCTAGAGACTGCCATATCTGGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605172359_brsn AGTGTTGGCCTATAGGCTGTACAAATATCAAG(+CAGTGT)GTACCCGGAGTAGTTACTCCTCTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605172357_f4i6 TTTTTTCTGAAGAAGAGGAGTAACTACTCCGG(+CAGTGT)GTACCTTGATATTTGTACAGCCTATAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605172356_qjyy AAGATGGGGCTCAAGAAATGGTCAGCTCTTGG(+CAGTGT)GTACCTAAGTATTTGAACAGAAGCTGCCACA NGG 10 8 17 8 24 0 100 5 10
    240605172354_95bz GCTTTTGTGGCAGCTTCTGTTCAAATACTTAG(+CAGTGT)GTACCCAAGAGCTGACCATTTCTTGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605172353_7g1l CAAGCCTTCGGCAGAGGGTCATGTACCACTGG(+CAGTGT)GATCCCAAGTACGGCTAGACCCACCAAAAAG NGG 10 8 17 8 24 0 100 5 10