Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173325_rjj9 CTGCCCCTCTGACTCCTGCCCAGAAGTGGTGG(+CAGTGT)GTACCCCCCCACCCCGTGCCATTCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605173323_7o5h GGGTGGGACAATGAATGGCACGGGGTGGGGGG(+CAGTGT)GTACCCACCACTTCTGGGCAGGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605173321_prfw GAGCTTCTTTGGTATCACCTGTCTATGTGCAG(+CAGTGT)GATCCTTCCAAAATGGAAGTAAACTACGCAG NGG 10 8 17 8 24 0 100 5 10
    240605173320_3tc0 GGGTGCTGCGTAGTTTACTTCCATTTTGGAAG(+CAGTGT)GATCCTGCACATAGACAGGTGATACCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605173318_5f97 TGTGTCTGGAACTCACCAAGGACACTAGCCCG(+CAGTGT)GATCCCTAGACAGAAGCTATGGACCCCATAA NGG 10 8 17 8 24 0 100 5 10
    240605173316_rhc2 GGGATTTATGGGGTCCATAGCTTCTGTCTAGG(+CAGTGT)GATCCGGGCTAGTGTCCTTGGTGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605173313_e7hw TCTGTTCAATAGAGGCCACTTCCTTCCCAAGG(+CAGTGT)GATCCAAAACCCAAAACCTGCGGCTTCCACT NGG 10 8 17 8 24 0 100 5 10
    240605173312_8bu3 GTTTCAGTGGAAGCCGCAGGTTTTGGGTTTTG(+CAGTGT)GATCCCTTGGGAAGGAAGTGGCCTCTATTGA NGG 10 8 17 8 24 0 100 5 10
    240605173310_ho9m AAGTCCATTTCCCAGCCATACGCAGCTGTGGG(+CAGTGT)GATCCCAGGAGACACTTCAAGTGGAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605173308_drxv TTGAGATTCATTCCACTTGAAGTGTCTCCTGG(+CAGTGT)GATCCCCACAGCTGCGTATGGCTGGGAAATG NGG 10 8 17 8 24 0 100 5 10