Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172351_350w TTGGCCTTTTTGGTGGGTCTAGCCGTACTTGG(+CAGTGT)GATCCCAGTGGTACATGACCCTCTGCCGAAG NGG 10 8 17 8 24 0 100 5 10
    240605172350_40os ATAAAGTTCAGTGCTATAGCACTTTGTGTGAG(+CAGTGT)GTACCTGGGTTCAACCCCAGCACTGCCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605172347_b5j3 GTGGGATGAGGCAGTGCTGGGGTTGAACCCAG(+CAGTGT)GTACCTCACACAAAGTGCTATAGCACTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605172346_vdpq GCCTGCGCTGGAGCAGTGCGAGCTTGTCCGTG(+CAGTGT)GTACCCGTACCCGCCCGCCCGCAGCGTGGCG NGG 10 8 17 8 24 0 100 5 10
    240605172344_w99n AATTGCGCCACGCTGCGGGCGGGCGGGTACGG(+CAGTGT)GTACCACGGACAAGCTCGCACTGCTCCAGCG NGG 10 8 17 8 24 0 100 5 10
    240605172343_p8tw CTTGGTTTCTCACTCCAGGCATCTTAGACTTG(+CAGTGT)GATCCTATTAAAAATCACATTCCCCAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605172342_5fr3 AAACCTGCCTTGGGGAATGTGATTTTTAATAG(+CAGTGT)GATCCAAGTCTAAGATGCCTGGAGTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605172340_7a5u TTCTCCTCTTTCCTCTCTCTAGGTCCTTCCAG(+CAGTGT)GTACCCTCCCTCCAACCCTTTCCATGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605172338_x2o0 AGCAGGGGAACATGGAAAGGGTTGGAGGGAGG(+CAGTGT)GTACCTGGAAGGACCTAGAGAGAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605172335_ttw8 GCTAGGGAAAAGGAGTGTTCGTTCTACCCAGG(+CAGTGT)GTACCACAGTTCCCCACAGTCAAAACCCAAA NGG 10 8 17 8 24 0 100 5 10