Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172334_0gnw TTGCTTTTGGGTTTTGACTGTGGGGAACTGTG(+CAGTGT)GTACCCTGGGTAGAACGAACACTCCTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605172332_uowu AGCCGCAGGCCGGGGGACCAGGGGTCGGCAGG(+CAGTGT)GTACCTCCCCAGAATACAGTAGATTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605172330_liq3 TTTATAGCTGAAATCTACTGTATTCTGGGGAG(+CAGTGT)GTACCCTGCCGACCCCTGGTCCCCCGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605172329_ni58 GCGAGGCCGCAGTCGCCCTCGGGGTTCTTAGG(+CAGTGT)GATCCCCAGCCTCTTGACAACAAGGCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605172327_bf5h GGGCCTGCCCGCCTTGTTGTCAAGAGGCTGGG(+CAGTGT)GATCCCTAAGAACCCCGAGGGCGACTGCGGC NGG 10 8 17 8 24 0 100 5 10
    240605172325_nj0m GAAGAGCAGCTTGCCACAGCTGGCCTACAATG(+CAGTGT)GTACCAGCATTCTCTTCGTGATCTCAACACA NGG 10 8 17 8 24 0 100 5 10
    240605172324_6r66 CACAGTGTGTTGAGATCACGAAGAGAATGCTG(+CAGTGT)GTACCATTGTAGGCCAGCTGTGGCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605172322_dvtp GCACAGACCTAGGGATGCACTCCCTGCCTTTG(+CAGTGT)GATCCCCACTCTACCATTTGCTAATTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605172320_0rok AAGGTCAACTAATTAGCAAATGGTAGAGTGGG(+CAGTGT)GATCCAAAGGCAGGGAGTGCATCCCTAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605172318_miyy TCAAATCCAAAGCCGGCCTGTGTGATGTAGTG(+CAGTGT)GTACCTCTTTCCATCTCAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10