Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605172317_kh53 GACTTTTTTTTTTTTTTTTGAGATGGAAAGAG(+CAGTGT)GTACCACTACATCACACAGGCCGGCTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605172316_g0w0 GAGGAGAGAAGACACTGGGGGCCTGGAGCTGG(+CAGTGT)GTACCAGGGCTACTACTGCCTTCCACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605172313_8auo TGGTCTCACAGTGGAAGGCAGTAGTAGCCCTG(+CAGTGT)GTACCCAGCTCCAGGCCCCCAGTGTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605172312_821t GAGGCCAGCCTGGGTTAGAGTGACATGTAGTG(+CAGTGT)GATCCTCATTATGTGTTCATGTTAGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605172311_n8rf TTGAGCTTTTTCTAACATGAACACATAATGAG(+CAGTGT)GATCCACTACATGTCACTCTAACCCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605172309_x0g7 CCATACCCTAAATGTATTCTATACTATACCTG(+CAGTGT)GTACCTCAGGGGGAAGGGATGCCAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605172307_f9xc GTGGACCCATGCTGGCATCCCTTCCCCCTGAG(+CAGTGT)GTACCAGGTATAGTATAGAATACATTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605172305_csqr GCCTATGGATATATGCATGGAGGTGTGTCTGG(+CAGTGT)GTACCTATTCACACAGGCTTACATATGACCA NGG 10 8 17 8 24 0 100 5 10
    240605172304_z6b2 GAGTGTGGTCATATGTAAGCCTGTGTGAATAG(+CAGTGT)GTACCCAGACACACCTCCATGCATATATCCA NGG 10 8 17 8 24 0 100 5 10
    240605172302_x5bs CGTTGGTCTTCAGTTCTGCAGGGAAGTTCTGG(+CAGTGT)GTACCTTTGGTTTTGTTTAAGGAGAAAAAAA NGG 10 8 17 8 24 0 100 5 10