Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165755_h81n AGAACAGACAATAGGTGCCAAGGTCAGACTTG(+CAGTGT)GTACCATCTTTGCCCTTGTACTGCTATCTTC NGG 10 8 17 8 24 0 100 5 10
    240605165753_2bl8 TTCTCTGTGTCTCAGGGATTGAGATGGGGTGG(+CAGTGT)GATCCTGCAAGTCACAGCCTGAGCTGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605165751_6wgo CCCCTACAGCCAGCTCAGGCTGTGACTTGCAG(+CAGTGT)GATCCCACCCCATCTCAATCCCTGAGACACA NGG 10 8 17 8 24 0 100 5 10
    240605165750_ts02 GGGAGCACAGGGAGGAGGCGTGTGAGGTTGAG(+CAGTGT)GATCCGTCTGCTGTGGATTCTTCTGTTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605165749_h0px AGCGCTGCAAACAGAAGAATCCACAGCAGACG(+CAGTGT)GATCCTCAACCTCACACGCCTCCTCCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605165746_232g GCTTCTCCCGGGAGGGCTGCCCAGAACCAGGG(+CAGTGT)GATCCTCCTCCTCCACAAACCCGAGGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240605165745_7pd7 GTGTCCCCTTCCTCGGGTTTGTGGAGGAGGAG(+CAGTGT)GATCCCCTGGTTCTGGGCAGCCCTCCCGGGA NGG 10 8 17 8 24 0 100 5 10
    240605165743_k6lx AGCTAGAGTACCCCTTCCCTCCCCACCACTGG(+CAGTGT)GATCCTTGATGGTACAGAGCAATTTTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605165742_6bok CTGCACAAGCAAAATTGCTCTGTACCATCAAG(+CAGTGT)GATCCCAGTGGTGGGGAGGGAAGGGGTACTC NGG 10 8 17 8 24 0 100 5 10
    240605165740_k6q0 TTTTTTTTTTTTTTTTTTTTTTTTAAACTTGG(+CAGTGT)GATCCCTAGGGCAGCTTGCACAGGAAATCCT NGG 10 8 17 8 24 0 100 5 10