Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165738_g05u ACCAAAGGATTTCCTGTGCAAGCTGCCCTAGG(+CAGTGT)GATCCCAAGTTTAAAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605165736_cj81 GCTAACAACTATCTGTAACTCTACTCTAAAGG(+CAGTGT)GATCCGATGATCTCTGGCTCTACTGGGCACT NGG 10 8 17 8 24 0 100 5 10
    240605165735_spq3 CATAAAGTGCCCAGTAGAGCCAGAGATCATCG(+CAGTGT)GATCCCTTTAGAGTAGAGTTACAGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605165733_w15b AAAATTGAAACTATAAACCAAGTACCCCACTG(+CAGTGT)GTACCCATCTTGTCTAAGCCCCTCTAACCTC NGG 10 8 17 8 24 0 100 5 10
    240605165731_8ov2 TGAGGGAGGTTAGAGGGGCTTAGACAAGATGG(+CAGTGT)GTACCAGTGGGGTACTTGGTTTATAGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605165730_10h0 GACTAGCTCATGTTAGCTCAGGCTCTGCTCAG(+CAGTGT)GATCCCAGCCAGCCCTGCTCTGCTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605165728_sr42 AAGACAGAGCAGAGCAGAGCAGGGCTGGCTGG(+CAGTGT)GATCCTGAGCAGAGCCTGAGCTAACATGAGC NGG 10 8 17 8 24 0 100 5 10
    240605165726_tyk8 CAGGGAAATACCCAATGTTGTTCTGCTCCCTG(+CAGTGT)GATCCCCGCAACTGTTTTCCCTCCCACTCTG NGG 10 8 17 8 24 0 100 5 10
    240605165724_z4sx TGCTCCAGAGTGGGAGGGAAAACAGTTGCGGG(+CAGTGT)GATCCAGGGAGCAGAACAACATTGGGTATTT NGG 10 8 17 8 24 0 100 5 10
    240605165721_0olc TTGCTGTCCTTGGTCTTACACAGAGAACCCAG(+CAGTGT)GTACCAGGCCACCTCCTTACTCAGAAGAGAG NGG 10 8 17 8 24 0 100 5 10