Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165719_2l8m GCAGACTCTCTTCTGAGTAAGGAGGTGGCCTG(+CAGTGT)GTACCTGGGTTCTCTGTGTAAGACCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605165716_kw4w AGCTCACAGGCACCTCCAACTCAAGCTCCGGG(+CAGTGT)GATCCAACCTCCTTGCTGGTTTCTTTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605165715_hspt CCTGATGCCCAAAGAAACCAGCAAGGAGGTTG(+CAGTGT)GATCCCCGGAGCTTGAGTTGGAGGTGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605165714_ebym GATCACAGATACTCAAAGAAGTGAGTGGTATG(+CAGTGT)GATCCACACATCCTCACAGTGTTATGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605165712_mbzg GGGAAATACACATAACACTGTGAGGATGTGTG(+CAGTGT)GATCCATACCACTCACTTCTTTGAGTATCTG NGG 10 8 17 8 24 0 100 5 10
    240605165711_r0km CATTCACTTAAGGGGTCTGGCTGCAGTGTGTG(+CAGTGT)GATCCGGACATTGAGGTCACAGAACAGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605165709_wqbl GTGCTGCTCCTGTTCTGTGACCTCAATGTCCG(+CAGTGT)GATCCACACACTGCAGCCAGACCCCTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605165707_6m8d CTTCCTGTACCTAAGGGCACTGGCTGGATTGG(+CAGTGT)GTACCGGGTAGCAGAAAAGAACGGGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605165704_29xc GCAGGCAGGCTCCCGTTCTTTTCTGCTACCCG(+CAGTGT)GTACCCAATCCAGCCAGTGCCCTTAGGTACA NGG 10 8 17 8 24 0 100 5 10
    240605165701_ss4m TCAAGCCTAGATTTGAGTTCTCCTTATCCCAG(+CAGTGT)GTACCTGGATTTATCCCTGCTGATTGTCAGT NGG 10 8 17 8 24 0 100 5 10