Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165640_jvs4 CCTTGGGCACAGAGTCTGCGGACCCCCACAAG(+CAGTGT)GTACCCACAGGACCCTCCATGGGATCTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605165638_y7hv GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605165636_b0ls GGTGTCAGCATTTGGAAGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605165634_grh0 CTTTAATGCCATCACTTGAGCAGAGACAGGTG(+CAGTGT)GATCCCTAAGTTTAAGTCCAGCCTCATCTAC NGG 10 8 17 8 24 0 100 5 10
    240605165633_8f2o GTTCTGTAGATGAGGCTGGACTTAAACTTAGG(+CAGTGT)GATCCACCTGTCTCTGCTCAAGTGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605165631_67xv CTTTAATGCCATCACTTGAGCAGAGACAGGTG(+CAGTGT)GATCCCTAAGTTTAAGTCCAGCCTCATCTAC NGG 10 8 17 8 24 0 100 5 10
    240605165630_1t0v GTTCTGTAGATGAGGCTGGACTTAAACTTAGG(+CAGTGT)GATCCACCTGTCTCTGCTCAAGTGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605165628_tojp TAATGGATAAGGCTTTATTTCTCTGCTGGAAG(+CAGTGT)GATCCATCTGGGAGAAAGAGAAGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605165627_em03 TATGGCCCCTGCCCTTCTCTTTCTCCCAGATG(+CAGTGT)GATCCTTCCAGCAGAGAAATAAAGCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240605165625_jit0 TCACAAAGGGGACCCCCTCCCAGGGCCTACAG(+CAGTGT)GATCCAGGGATTGAATGCTCAGGGAAGCACT NGG 10 8 17 8 24 0 100 5 10