Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165623_z0fh GTGGTAGTGCTTCCCTGAGCATTCAATCCCTG(+CAGTGT)GATCCTGTAGGCCCTGGGAGGGGGTCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605165621_u0mi ACTACCAAATTGCTTGGCCCTTACCCTTCCAG(+CAGTGT)GATCCCTGCATATCCTCTCCAGCCCCAACCC NGG 10 8 17 8 24 0 100 5 10
    240605165620_td2o GGGGTGGGTTGGGGCTGGAGAGGATATGCAGG(+CAGTGT)GATCCTGGAAGGGTAAGGGCCAAGCAATTTG NGG 10 8 17 8 24 0 100 5 10
    240605165618_565n TTGCTACTGGGCCACAGAATGGGTAACACGTG(+CAGTGT)GTACCAACTTTGATTTGGTCAAAGTTGATGT NGG 10 8 17 8 24 0 100 5 10
    240605165617_3u98 AGGCAACATCAACTTTGACCAAATCAAAGTTG(+CAGTGT)GTACCACGTGTTACCCATTCTGTGGCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605165616_ak5n TGCTACCCAAGGAGAGCATGCCAGATAGCGAG(+CAGTGT)GATCCAAGGTCAAGGCAGAGCCTTGACAGCT NGG 10 8 17 8 24 0 100 5 10
    240605165614_acgt CTCTTAGCTGTCAAGGCTCTGCCTTGACCTTG(+CAGTGT)GATCCTCGCTATCTGGCATGCTCTCCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605165612_17hz CTGCTGTGGGTTCTGACAGTGACTGAGGCCTG(+CAGTGT)GATCCCCACCCTGCCCCAGCCTGAGAAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605165610_5p6v CATGGACCTTTCTCAGGCTGGGGCAGGGTGGG(+CAGTGT)GATCCAGGCCTCAGTCACTGTCAGAACCCAC NGG 10 8 17 8 24 0 100 5 10
    240605165608_lhxj ACATTCTCAGTTATTTCCTATTGGCAGCTGAG(+CAGTGT)GATCCACTGCAATCTGCATGTGATCATTGAA NGG 10 8 17 8 24 0 100 5 10