Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163639_xdko GCCCCGCTCCGAGGGCCTCGGCGATGAACCTG(+CAGTGT)GATCCCTGGTTTTCTCCAAGAAGGACTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605163637_ksfv CATTGTGCCTCTGAGACATCCTTAGGGCTGGG(+CAGTGT)GTACCCAGCTCTCGAAGACACCATGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240605163636_538i AGAAGTCATGGCATGGTGTCTTCGAGAGCTGG(+CAGTGT)GTACCCCAGCCCTAAGGATGTCTCAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605163634_f7gk ACTAACATCTCCCTCTCCCTCTCTTGCTATGG(+CAGTGT)GATCCCAATGGTACTGCAGGCAACACAGTAC NGG 10 8 17 8 24 0 100 5 10
    240605163632_c9lr GACTGGTACTGTGTTGCCTGCAGTACCATTGG(+CAGTGT)GATCCCATAGCAAGAGAGGGAGAGGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240605163629_r827 GGCTCTTCTGGCTTTTACCTTCACCGATGGGG(+CAGTGT)GTACCTGGTGTCCTGAGGTGGCCAAGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605163627_3n5x TGTAGGCTCACTTGGCCACCTCAGGACACCAG(+CAGTGT)GTACCCCCATCGGTGAAGGTAAAAGCCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605163626_np6r CAGGTGGATGTTTGGGTTCTGTTGGTAACTGG(+CAGTGT)GTACCTGCAAAAGAGCTTAGGACACTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605163625_1gan TAATGAGCAAAGTGTCCTAAGCTCTTTTGCAG(+CAGTGT)GTACCCAGTTACCAACAGAACCCAAACATCC NGG 10 8 17 8 24 0 100 5 10
    240605163623_deaj TCCAGCATGAGCAGGCTCCCTAGCTGTTGAAG(+CAGTGT)GATCCCATGGGTCAGGCACTGACCACCTTGA NGG 10 8 17 8 24 0 100 5 10