Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163605_7ov5 GGTGATGGAAGGAATGGTTCAAAAGAGGGGAG(+CAGTGT)GATCCTCAGATCAATACAGCCAGAGACATTT NGG 10 8 17 8 24 0 100 5 10
    240605163604_xepf CAAGTCTGTGCCTCAGCACAGCTAGAAGTCAG(+CAGTGT)GATCCAAGGAGGCCTAAAATCGGCAATGACA NGG 10 8 17 8 24 0 100 5 10
    240605163602_ytu3 GAGTCTGTCATTGCCGATTTTAGGCCTCCTTG(+CAGTGT)GATCCTGACTTCTAGCTGTGCTGAGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240605163600_g14h CTCCCCATGGATTCATGTGCACCCATCTGGAG(+CAGTGT)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605163559_the4 TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+CAGTGT)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    240605163557_4ftw CCCCATGCTACTGATAACACAGTGATCACCAG(+CAGTGT)GATCCAAACTGGCTTCAGGTTTCTAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240605163555_q6q8 GACGCTTTTGTTAGAAACCTGAAGCCAGTTTG(+CAGTGT)GATCCTGGTGATCACTGTGTTATCAGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240605163554_hqbo ACTCCATAGATCTCAGTAATTCTGGAAACAAG(+CAGTGT)GTACCATGACATGCAGGTCATCATTGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605163553_kcsg TTTGGTTTTCCAATGATGACCTGCATGTCATG(+CAGTGT)GTACCTTGTTTCCAGAATTACTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240605163551_2d1r AAAAGGATATACCTTCTTCTCAGCACCTCATG(+CAGTGT)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10