Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163621_yykw CACCTTCAAGGTGGTCAGTGCCTGACCCATGG(+CAGTGT)GATCCTTCAACAGCTAGGGAGCCTGCTCATG NGG 10 8 17 8 24 0 100 5 10
    240605163619_t8a9 CTATGATCATATAATAACAACAATGTAGAAGG(+CAGTGT)GTACCACACCCTTGCTTGCCATGGCCTCACC NGG 10 8 17 8 24 0 100 5 10
    240605163618_t8ja GAGATGGTGAGGCCATGGCAAGCAAGGGTGTG(+CAGTGT)GTACCCTTCTACATTGTTGTTATTATATGAT NGG 10 8 17 8 24 0 100 5 10
    240605163616_j0u1 GTTCCACTTAGTCAGGAAGTCTCCAGCCTATG(+CAGTGT)GATCCTGTGCCACCCCCTGCCCCCAACGCCC NGG 10 8 17 8 24 0 100 5 10
    240605163614_j41y TGTTTGGGCGTTGGGGGCAGGGGGTGGCACAG(+CAGTGT)GATCCATAGGCTGGAGACTTCCTGACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605163613_mtyg TGAGATGAGTCCACCTCATCTGTAGCCTAGAG(+CAGTGT)GTACCTCAGTGAGTTGGGAAAGTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605163612_q82u TAAGTGGCCTCTGACTTTCCCAACTCACTGAG(+CAGTGT)GTACCTCTAGGCTACAGATGAGGTGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240605163610_z371 TCGCTGGGGCTGCCCCATAGGACACTGGGGTG(+CAGTGT)GTACCATGGAGAGACAGGGGATAAAACTGAT NGG 10 8 17 8 24 0 100 5 10
    240605163608_rxhb TTTGGATCAGTTTTATCCCCTGTCTCTCCATG(+CAGTGT)GTACCACCCCAGTGTCCTATGGGGCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605163607_2k0l GCTCAAAATGTCTCTGGCTGTATTGATCTGAG(+CAGTGT)GATCCTCCCCTCTTTTGAACCATTCCTTCCA NGG 10 8 17 8 24 0 100 5 10