Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163518_bsy6 CTCTTTGGGAGGTTCTCAGGACCCAAAGGAAG(+CAGTGT)GTACCTTTGATGAAATCTCCTACAGTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605163517_db8q AGTATTAGGCCTTGGGGCCTCCCCTTGAGCTG(+CAGTGT)GATCCCAATTTGGGCCTGTCGCTGGACCTCT NGG 10 8 17 8 24 0 100 5 10
    240605163516_cwaf GAAAAAGAGGTCCAGCGACAGGCCCAAATTGG(+CAGTGT)GATCCAGCTCAAGGGGAGGCCCCAAGGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605163514_jt32 AGAGGCATTATTGTTTCTTTCCACCTTACAGG(+CAGTGT)GTACCTCCCTTTATTCCTTCCTTCCTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605163512_suvd GAAGGAAGGAAGGAAGGAAGGAATAAAGGGAG(+CAGTGT)GTACCCTGTAAGGTGGAAAGAAACAATAATG NGG 10 8 17 8 24 0 100 5 10
    240605163510_jz3r CATGCACGCGCGCCACCACGCCCGGCTACAGG(+CAGTGT)GTACCTTTCAATAAATGTGGATTTCCAAACA NGG 10 8 17 8 24 0 100 5 10
    240605163508_f4ad ATTCAGGGTCAGCTTCAACTCCAGTTGTTTGG(+CAGTGT)GTACCTATATAAAGACCAAGTTGCACATCTG NGG 10 8 17 8 24 0 100 5 10
    240605163507_03oq TGTAGCAGATGTGCAACTTGGTCTTTATATAG(+CAGTGT)GTACCCAAACAACTGGAGTTGAAGCTGACCC NGG 10 8 17 8 24 0 100 5 10
    240605163505_p7pr TCACAGCTAGGGTAAACTCCACTGATTCTTGG(+CAGTGT)GTACCTCCTTTATCCCAGTGGTTTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605163504_8vxb AAACAGAAAAAAAAACCACTGGGATAAAGGAG(+CAGTGT)GTACCCAAGAATCAGTGGAGTTTACCCTAGC NGG 10 8 17 8 24 0 100 5 10