Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163533_qtbi ACTCCATAGATCTCAATAATTCTGGAAACAAG(+CAGTGT)GTACCATGACATGCAGGTCATCATCGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605163532_gxmh ACTCCATAGATCTCAATAATTCTGGAAACAAG(+CAGTGT)GTACCATGACATGCAGGTCATCATCGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605163531_jh3w TTTGGTTTTCCGATGATGACCTGCATGTCATG(+CAGTGT)GTACCTTGTTTCCAGAATTATTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240605163529_vaaq AACAAAAAACAAAAAACACTGGGATAAAGGAG(+CAGTGT)GTACCCAAGAATCAGTGGAGTTTACCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605163527_fzyr TCACAGCTAGGGTAAACTCCACTGATTCTTGG(+CAGTGT)GTACCTCCTTTATCCCAGTGTTTTTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605163526_v9cg CAGGAATAGAAATCCTGACCAAGACATTGTTG(+CAGTGT)GTACCAGCATAGTGGGGCATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240605163525_2jey TCAGGTTGTCACAGGAATGCCCCACTATGCTG(+CAGTGT)GTACCAACAATGTCTTGGTCAGGATTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605163523_ds1d AGTGATGAAAAGGAGAGGGCCCCTTGTGGGTG(+CAGTGT)GTACCATCTCTGGGCTGGTAATATTGGATTC NGG 10 8 17 8 24 0 100 5 10
    240605163521_eck5 TTATAGAATCCAATATTACCAGCCCAGAGATG(+CAGTGT)GTACCACCCACAAGGGGCCCTCTCCTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605163520_ww3u CCTTCTCCCCACTGTAGGAGATTTCATCAAAG(+CAGTGT)GTACCTTCCTTTGGGTCCTGAGAACCTCCCA NGG 10 8 17 8 24 0 100 5 10