Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163502_mm3v ATCGACAGATGAACCAAGAGAATAGGAAAGAG(+CAGTGT)GATCCCAAAGCCGAACAAGAAAATCACGATC NGG 10 8 17 8 24 0 100 5 10
    240605163500_5vwb TAAGGGATCGTGATTTTCTTGTTCGGCTTTGG(+CAGTGT)GATCCTCTTTCCTATTCTCTTGGTTCATCTG NGG 10 8 17 8 24 0 100 5 10
    240605163459_p4h1 CAGAAAGGGGTCCCCTTTAGTGAAATGCTGGG(+CAGTGT)GATCCTTCTACAGCTCATGCTGGTTCACTTG NGG 10 8 17 8 24 0 100 5 10
    240605163457_ey9y CTTCCCAAGTGAACCAGCATGAGCTGTAGAAG(+CAGTGT)GATCCCCAGCATTTCACTAAAGGGGACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605163456_lcpj GGTGTCATCGTTTTTAGGCTGATTATGGGATG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605163453_wpcx GGATGGACCATCTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCATCCCATAATCAGCCTAAAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240605163452_b9zd TTATGCTCCTCTAATTCTGCATTCTCAGGCAG(+CAGTGT)GATCCATCTGTGGACAGAGACAGAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605163450_z1r9 GCTGGGAGATCTTCTGTCTCTGTCCACAGATG(+CAGTGT)GATCCTGCCTGAGAATGCAGAATTAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605163449_ogb3 AGAGATCCACCATCCTCTTTTTGCCTCCACTG(+CAGTGT)GTACCAGGCATGCATGTGATGCTCACGCATA NGG 10 8 17 8 24 0 100 5 10
    240605163447_ocve GCATGTATGCGTGAGCATCACATGCATGCCTG(+CAGTGT)GTACCAGTGGAGGCAAAAAGAGGATGGTGGA NGG 10 8 17 8 24 0 100 5 10