Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125353_rnn9 AAAACAGTTGTTTCCTATACAGAAATCTCAGG(+CAGTGT)GTACCTTTAGATTTAATTACGTTTATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605125352_donh AGGTTTCTAGAAACACGCAGACACTGAGAATG(+CAGTGT)GTACCCAGGGTCCTGCTCTTTACCACAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605125349_6ck5 GCATGAACCTGTGGTAAAGAGCAGGACCCTGG(+CAGTGT)GTACCATTCTCAGTGTCTGCGTGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605125348_ozpz AGAGGTTAGATGTTCTTGGCCTGCAGGGACAG(+CAGTGT)GTACCTATAGTGTTGGCATAGTCAGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605125347_0tc5 TGCTGGGAAACCTGACTATGCCAACACTATAG(+CAGTGT)GTACCTGTCCCTGCAGGCCAAGAACATCTAA NGG 10 8 17 8 24 0 100 5 10
    240605125345_i3iw TGCCGTCGGGCTGGATCCTAGGTGGACACTTG(+CAGTGT)GTACCTCTCTGTTGCTGTTTCTTGGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605125344_r38k AGCTGGGCTCTCCAAGAAACAGCAACAGAGAG(+CAGTGT)GTACCAAGTGTCCACCTAGGATCCAGCCCGA NGG 10 8 17 8 24 0 100 5 10
    240605125343_eyg8 CCCTAAGGAGACGTCAGGGTGCCGTCGGGCTG(+CAGTGT)GATCCTAGGTGGACACTTGGTACCTCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605125341_gy8x CAGCAACAGAGAGGTACCAAGTGTCCACCTAG(+CAGTGT)GATCCAGCCCGACGGCACCCTGACGTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605125338_tdh6 CCTGGCCTACAATCCCTTGTCAGGCGTCCAGG(+CAGTGT)GTACCACACTGAGAACAGCTGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10