Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125439_ry64 ATTTATACTCTACTGGAAGTTTTTTTTTTAAG(+CAGTGT)GTACCCTACAGCAGCTGCTTAAAATGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605125436_b5f4 CTCGAGAAACGCAGTCTCGGCCTCCTCCCAGG(+CAGTGT)GATCCAAACGCCAGCCACCCGCCCGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605125434_5ywi TCTCTAAAAGCCGGGCGGGTGGCTGGCGTTTG(+CAGTGT)GATCCCTGGGAGGAGGCCGAGACTGCGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605125433_7qpp GACTGTGAATGAAGAGCTGTGCTGTGTGGGTG(+CAGTGT)GTACCTGCTGTCTCCTATCTGCTGGGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605125432_twtp AGATCTAAGACCCAGCAGATAGGAGACAGCAG(+CAGTGT)GTACCACCCACACAGCACAGCTCTTCATTCA NGG 10 8 17 8 24 0 100 5 10
    240605125431_kg14 GATGTTGCTGCTGTTAGGAAGGTAGGCAGAAG(+CAGTGT)GATCCATGTCTGTGCCGAGAGGTGTGACGCC NGG 10 8 17 8 24 0 100 5 10
    240605125429_fhp9 CCCCAGGCGTCACACCTCTCGGCACAGACATG(+CAGTGT)GATCCTTCTGCCTACCTTCCTAACAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605125428_vsdj CTCCTGTCGGGTATTTCTTACTGGCACAGTAG(+CAGTGT)GTACCTGGATGTTCACAGAGCATCCGCCCCG NGG 10 8 17 8 24 0 100 5 10
    240605125427_u5dy TGGGACGGGGCGGATGCTCTGTGAACATCCAG(+CAGTGT)GTACCTACTGTGCCAGTAAGAAATACCCGAC NGG 10 8 17 8 24 0 100 5 10
    240605125425_42hq ATATCAGCAATGGCTTTTATTTAAGGATTGAG(+CAGTGT)GTACCACGAAGATGTGGGAGGAGCTGTTGAT NGG 10 8 17 8 24 0 100 5 10