Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125512_4be4 AGGGTTCCGGCACCGAAGGGAAGGGCGGCAAG(+CAGTGT)GATCCTAGCCCTTCCGCACCAACCTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240605125510_03b3 AGGCAGGTTCTCTCTGCTGTCCTCAGGCAGTG(+CAGTGT)GTACCCTTGGGGGTCTCCTTGGTCTTCACAC NGG 10 8 17 8 24 0 100 5 10
    240605125508_hyc8 CGTGTGTGTGAAGACCAAGGAGACCCCCAAGG(+CAGTGT)GTACCACTGCCTGAGGACAGCAGAGAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605125506_3rbh GCCACGGCTTGCGATGTATAAGCACACAGAAG(+CAGTGT)GATCCAGTGCAATAAAGACGTGAAACGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605125505_h279 ACACACACACGTTTCACGTCTTTATTGCACTG(+CAGTGT)GATCCTTCTGTGTGCTTATACATCGCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605125502_bvjc TGAAACCACTCAAAGTCACAAAGAAACCCAGG(+CAGTGT)GATCCCCTAAGCTGTGGTCCAGGTGCCCTAG NGG 10 8 17 8 24 0 100 5 10
    240605125500_rlub AAGTACTAGGGCACCTGGACCACAGCTTAGGG(+CAGTGT)GATCCCTGGGTTTCTTTGTGACTTTGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605125458_05yy CACTGGAGGTGCAGGTGCCTGGGCCAGCCTTG(+CAGTGT)GATCCATATCCAGTATTTGGCAGATTCATGC NGG 10 8 17 8 24 0 100 5 10
    240605125457_3e23 GGCAGGCATGAATCTGCCAAATACTGGATATG(+CAGTGT)GATCCAAGGCTGGCCCAGGCACCTGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240605125456_gf9a TGCCCATCGCTAAAGCTAAGACCTGCAGTAAG(+CAGTGT)GATCCCTCAGAGCCTCTTTTCAAAGCATGGT NGG 10 8 17 8 24 0 100 5 10