Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605125600_pvr5 TTGCCTGCATGTGTGTCTTTATGAGGGTGTTG(+CAGTGT)GATCCCCTGAAATTGGAGTTACAGATAATTA NGG 10 8 17 8 24 0 100 5 10
    240605125558_h8zk AGTGTCTGGGTTTGGTGGTTGTTTATGGGATG(+CAGTGT)GATCCCCAGGTGGGGCAGTCTTTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605125556_07vr GGAAGGACCATCCAAAGACTGCCCCACCTGGG(+CAGTGT)GATCCATCCCATAAACAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605125555_op4u GGATTAAGGTACTATGCCAAGCGTTTAAAGTG(+CAGTGT)GATCCTGTGGATAAACTTAGCTTCAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605125553_yozr ACCTGTGTTCTTGAAGCTAAGTTTATCCACAG(+CAGTGT)GATCCACTTTAAACGCTTGGCATAGTACCTT NGG 10 8 17 8 24 0 100 5 10
    240605125551_gijy TGAGGTGTTCTGGAGGGAATGGGCATGACAGG(+CAGTGT)GTACCTTAGGCTGAAAGCCAGCAAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240605125550_t72t CATCCTGTGCCTTTGCTGGCTTTCAGCCTAAG(+CAGTGT)GTACCCTGTCATGCCCATTCCCTCCAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605125547_2fxu TGGAGGCCCCAGGGCCAGAGTCCAAAGCCAGG(+CAGTGT)GTACCAGCTGCAGGAGATGGAGAAGGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605125546_53lm CTCCCTGGCCCCTTCTCCATCTCCTGCAGCTG(+CAGTGT)GTACCCTGGCTTTGGACTCTGGCCCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605125545_urdc ACCGTGTGATAAGGTTGTGTGCTGTGTGATCG(+CAGTGT)GTACCCAGGGAGCGGGGCGGTGTTAATGTGC NGG 10 8 17 8 24 0 100 5 10